The dual role of phytoene synthase genes in carotenogenesis in carrot roots and leaves

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• 作者：Hui Wang (1)
  Cheng-Gang Ou (1)
  Fei-Yun Zhuang (1)
  Zhen-Guo Ma (1)
  
• 关键词：Carrot ; Backcross inbred lines (BILs) ; Phytoene synthase gene ; Carotenoids accumulation ; De ; etiolation
• 刊名：Molecular Breeding
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：34
• 期：4
• 页码：2065-2079
• 全文大小：776 KB
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• 作者单位：Hui Wang (1)
  Cheng-Gang Ou (1)
  Fei-Yun Zhuang (1)
  Zhen-Guo Ma (1)
  
  1. Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, No. 12 Nanda Street, Zhongguan Cun, Haidian District, Beijing, 100081, China
  
• ISSN：1572-9788

文摘

Carrot (Daucus carota L.) is an important food crop and is useful for studying carotenogenesis due to the quantity and diversity of carotenoids in its roots. Phytoene synthase catalyzes the first committed step in the carotenoid biosynthesis pathway, and its overexpression is the main driving force in the orange phenotype. At present, we lack fundamental knowledge of the role of these genes and their effects on carotenoid accumulation in leaves. In the present study, three backcross inbred lines (BC2S4) with different colored roots derived from a cross between the orange inbred line (Af) and related wild species were used to investigate the role of the duplicated DcPSY genes in root carotenogenesis. Promoter analysis showed that DcPSY genes have diverged substantially in their regulatory sequences after gene duplication. Expression levels of DcPSY1 and DcPSY2 were generally positively correlated with carotenoid content during root development. In mature leaves, total carotenoid content was higher than that in the roots, DcPSY1 expression increased extremely higher than DcPSY2 expression compared with roots, and DcPSY1 was more sensitive than DcPSY2 during leaf de-etiolation under sunlight. These results suggest that DcPSY1 seems to make an important contribution to carotenoid accumulation in the leaves and is important for photosynthesis and photoprotection, but they are not the determining factors of root color. This expands our understanding of the regulation of carotenoid biosynthesis in carrot.