Factors contributing to the recalcitrance of herbaceous dicotyledons (forbs) to enzymatic deconstruction

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• 作者：Dina Jabbour ; Evan R Angelos ; Achira Mukhopadhyay…
• 关键词：Milkweed ; Goldenrod ; Queen Anne’s lace ; Lamb’s quarters ; Cellulase ; Biofuels ; Pretreatment ; Alkaline hydrogen peroxide ; AFEX
• 刊名：Biotechnology for Biofuels
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：569 KB
• 参考文献：1. Carroll, A, Somerville, C (2009) Cellulosic biofuels. Annu Rev Plant Biol 60: pp. 165-182 CrossRef
  2. Robertson, GP, Dale, VH, Doering, OC, Hamburg, SP, Melillo, JM, Wander, MM, Parton, WJ, Adler, PR, Barney, JN, Cruse, RM, Duke, CS, Fearnside, PM, Follett, RF, Gibbs, HK, Goldemberg, J, Mladenoff, DJ, Ojima, D, Palmer, MW, Sharpley, A, Wallace, L, Weathers, KC, Wiens, JA, Wilhelm, WW (2008) Sustainable biofuels redux. Science 322: pp. 49-50 CrossRef
  3. Tilman, D, Scolow, R, Foley, JA, Hill, J, Larson, E, Lynd, L, Pacala, S, Reilly, J, Searchinger, T, Somerville, C, Williams, R (2009) Beneficial biofuels - The food, energy, and environment trilemma. Science 325: pp. 270-271 CrossRef
  4. Landis, DA, Gardiner, MM, van der Werf, W, Swinton, SM (2008) Increasing corn for biofuel production reduces biocontrol services in agricultural landscapes. Proc Natl Acad Sci 105: pp. 20552-20557 CrossRef
  5. Davis, SC, Anderson-Teixeira, KJ, Delucia, EH (2009) Life-cycle analysis and the ecology of biofuels. Trends Plant Sci 14: pp. 140-146 CrossRef
  6. Fischer, G, Prieler, S, van Velthuizen, HT (2010) Biofuel production potentials in Europe: sustainable use of surplus cultivated land and pastures part II: land use scenarios. Biomass Bioenergy 34: pp. 173-187 CrossRef
  7. Garlock, RJ, Bals, B, Jasrotia, P, Balan, V, Dale, BE (2012) Influence of variable species composition on the saccharification of AFEX pretreated biomass from unmanaged fields in comparison to corn stover. Biomass Bioenergy 37: pp. 49-59 CrossRef
  8. Gelfand, I, Zenone, T, Jasrotia, P, Jiquan Chen, J, Hamilton, SK, Robertson, GP (2011) Carbon debt of Conservation Reserve Program (CRP) grasslands converted to bioenergy production. Proc Natl Acad Sci USA 108: pp. 13864-13869 CrossRef
  9. Gelfand, I, Sahajpal, R, Zhang, X, Izaurralde, RC, Gross, KL, Robertson, GP (2013) Sustainable bioenergy production from marginal lands in the US Midwest. Nature 93: pp. 514-517 CrossRef
  10. James, LA, Swinton, SM, Thelen, KD (2010) Profitability analysis of cellulosic energy crops compared to corn. Agron J 102: pp. 675-687 CrossRef
  11. Jarchow, ME, Liebman, M, Rawat, V, Anex, RP (2012) Functional group and fertilization affect the composition and bioenergy yields of prairie plants. GCB Bioenergy 4: pp. 671-679 CrossRef
  12. Meehan, TD, Hurlbert, AH, Gratton, C (2010) Bird communities in future bioenergy landscapes of the Upper Midwest. Proc Natl Acad Sci USA 43: pp. 18533-18538 CrossRef
  13. Tilman, D, Hill, J, Lehman, C (2006) Carbon-negative biofuels from low-input high-diversity grassland biomass. Science 314: pp. 1598-1600 CrossRef
  14. Wiens, J, Fargione, J, Hill, J (2011) Biofuels and biodiversity. Ecol Appl 21: pp. 1085-1095 CrossRef
  15. Banerjee, G, Car, S, Liu, T, Williams, DL, Meza, SL, Walton, JD, Hodge, DB (2012) Scale-up and integration of alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis, and ethanolic fermentation. Biotechnol Bioeng 109: pp. 922-931 CrossRef
  16. Banerjee, G, Car, S, Scott-Craig, JS, Hodge, D, Walton, JD (2011) Alkaline peroxide pretreatment of corn stover: effects of biom
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Plant Breeding/Biotechnology
  Renewable and Green Energy
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1754-6834

文摘

Background Many different feedstocks are under consideration for the practical production of biofuels from lignocellulosic materials. The best choice under any particular combination of economic, agronomic, and environmental conditions depends on multiple factors. The use of old fields, restored prairie, or marginal lands to grow biofuel feedstocks offers several potential benefits including minimal agronomic inputs, reduced competition with food production, and high biodiversity. However, a major component of such landscapes is often herbaceous dicotyledonous plants, also known as forbs. The potential and obstacles of using forbs as biofuel feedstocks compared to the more frequently considered grasses and woody plants are poorly understood. Results The factors that contribute to the yield of fermentable sugars from four representative forbs were studied in comparison with corn stover. The forbs chosen for the study were lamb’s quarters (Chenopodium album), goldenrod (Solidago canadensis), milkweed (Asclepias syriaca), and Queen Anne’s lace (Daucus carota). These plants are taxonomically diverse, widely distributed in northern temperate regions including the continental United States, and are weedy but not invasive. All of the forbs had lower total glucose (Glc) content from all sources (cell walls, sucrose, starch, glucosides, and free Glc) compared to corn stover (range 16.2 to 23.0% on a dry weight basis compared to 39.2% for corn stover). When digested with commercial enzyme mixtures after alkaline pretreatment, yields of Glc as a percentage of total Glc were lower for the forbs compared to corn stover. Enzyme inhibition by water-extractable compounds was not a significant contributor to the lower yields. Based on experiments with optimized cocktails of pure glycosyl hydrolases, enzyme imbalance probably accounted for much of the lower yields. Addition of xyloglucanase and α-xylosidase, two enzymes targeting Glc-containing polysaccharides that are more abundant in dicotyledonous plants compared to grasses, enhanced Glc yields from lamb’s quarters, but Glc yields were still lower than from corn stover. Conclusion The potential utilization of forb-rich plant communities as biofuel feedstocks must take into account their lower Glc content compared to grasses such as corn stover. Furthermore, new enzyme mixtures tailored to the different cell wall composition of forbs will have to be developed.