17β-Estradiol protects against apoptosis induced by interleukin-1β in rat nucleus pulposus cells by down-regulating MMP-3 and MMP-13
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  • 作者：Si-Dong Yang ; Da-Long Yang ; Ya-Peng Sun ; Bao-Lin Wang ; Lei Ma ; Shi-Qing Feng…
  • 关键词：17β ; estradiol ; Apoptosis ; Interleukin ; 1β ; Nucleus pulposus ; Matrix metalloproteinase ; Intervertebral disc degeneration
  • 刊名：Apoptosis
  • 出版年：2015
  • 出版时间：March 2015
  • 年：2015
  • 卷：20
  • 期：3
  • 页码：348-357
  • 全文大小：3,257 KB
  • 参考文献：1. Park JB, Kim KW, Han CW, Chang H (2001) Expression of Fas receptor on disc cells in herniated lumbar disc tissue. Spine 26:142-46 CrossRef
    2. Antoniou J, Steffen T, Nelson F, Winterbottom N, Hollander AP, Poole RA, Aebi M, Alini M (1996) The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration. J Clin Invest 98:996-003 CrossRef
    3. Buckwalter JA (1995) Aging and degeneration of the human intervertebral disc. Spine 20:1307-314
    4. Zhou GQ, Yang F, Leung VYL (2008) Molecular and cellular biology of the intervertebral disc and the use of animal models. Current Orthop 22:267-73 CrossRef
    5. Le Maitre CL, Freemont AJ, Hoyland JA (2007) Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration. Arthritis Res Ther 9:R45 CrossRef
    6. Alini M, Roughley PJ, Antoniou J, Stoll T, Aebi M (2002) A biological approach to treating disc degeneration: not for today, but may be for tomorrow. Eur Spine J 11:S215–S220
    7. Kroeber M, Unglaub F, Guehring T, Nerlich A, Hadi T, Lotz J, Carstens C (2005) Effects of controlled dynamic disc distraction on degenerated intervertebral discs: an in vivo study on the rabbit lumbar spine model. Spine 30:181-87 CrossRef
    8. Zhao CQ, Liu D, Li H, Jiang LS, Dai LY (2007) Interleukin-1β enhances the effect of serum deprivation on rat annular cell apoptosis. Apoptosis 12:2155-161 CrossRef
    9. Yang D, Wang D, Shimer A, Shen FH, Li X, Yang X (2014) Glutathione protects human nucleus pulposus?cells?from cell?apoptosis?and inhibition of matrix synthesis. Connect Tissue Res 55(2):132-39. doi:10.3109/03008207.2013.876421 CrossRef
    10. Zhang CC, Cui GP, Hu JG, Xiao YZ, Zhou XS, Shao C, Lin Q, Zhou JS (2014) Effects of adenoviral vector expressing hIGF-1 on?apoptosis?in nucleus pulposus?cells?in vitro. Int J Mol Med 33(2):401-05. doi:10.3892/ijmm.2013.1586
    11. Park JB, Park IC, Park SJ, Jin HO, Lee JK, Riew KD (2006) Anti-apoptotic effects of caspase inhibitors on rat intervertebral disc cells. J Bone Joint Surg Am 88:771-79 CrossRef
    12. Portt L, Norman G, Clapp C, Greenwood M (1813) Greenwood MT (2011) Anti-apoptosis?and cell survival: a review. Biochim Biophys Acta 1:238-59. doi:10.1016/j.bbamcr.2010.10.010
    13. Duprez L, Wirawan E, Vanden Berghe T, Vandenabeele P (2009) Major cell death pathways at a glance. Microbes Infect 11:1050-062 CrossRef
    14. Gupta S, Kass GE, Szegezdi E, Joseph B (2009) The mitochondrial death pathway: a promising therapeutic target in Diseases. J Cell Mol Med 13:1004-033 CrossRef
    15. Brunelle JK, Letai A (2009) Control of mitochondrial apoptosis by the Bcl-2 family. J Cell Sci 122:437-41 CrossRef
    16. Galluzzi L, Morselli E, Kepp O, Vitale I, Rigoni A, Vacchelli E, Michaud M, Zischka H, Castedo M, Kroemer G (2010) Mitochondrial gateways to cancer. Mol Aspects Med 31:1-0 CrossRef
    17. Mavrogonatou E, Angelopoulou MT, Kletsas D (2014) The catabolic effect of TNFα on bovine nucleus pulposus intervertebral?disc?cells and the restraining role of glucosamine sulfate in the TNFα-mediated up-regulation of?MMP-3. J Orthop Res 32(12):1701-707. doi:10.1002/jor.22725
文摘
In our previous study, 17β-estradiol was proved to protect rat annulus fibrosus cells against apoptosis induced by interleukin-1β (IL-1β). However, whether 17β-estradiol has protective effect on rat nucleus pulposus cells remains unclear. The purpose of this study was to further explore the effects of 17β-estradiol on rat nucleus pulposus cells based on IL-1β-induced apoptosis. TUNEL assay and Annexin V/PI double staining were used to detect apoptosis and revealed that IL-1β induced notable apoptosis, which was reversed by 17β-estradiol. Meanwhile, cell viability and binding ability were decreased by IL-1β, but activated caspase-3 was increased. However, all of the detected effects of IL-1β were eliminated by 17β-estradiol. Furthermore, real-time quantitative RT-PCR was used to further find that IL-1β downregulated expression level of type II collagen, aggrecan, tissue inhibitor of matrix metalloproteinase (TIMP)-1, while upregulated matrix metalloproteinase (MMP)-3, MMP-13 and Bcl-2, which was further confirmed by western blot. Finally, 17β-estradiol was proved to abolish the above negative effects of IL-1β. In summary, this work presented that IL-1β maybe induced apoptosis of rat nucleus pulposus cells, which was resisted by 17β-estradiol by down-regulating MMP-3 and MMP-13 via a mitochondrial pathway. This research provides a novel insight into the anti-apoptotic effect of 17β-estradiol on IL-1β-induced cytotoxicity, and may potentially lead to a better understanding of the clinical effects of 17β-estradiol, especially in terms of intervertebral disc degeneration.
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