Knockdown of Cellular RNA Helicase DDX3 by Short Hairpin RNAs Suppresses HIV-1 Viral Replication Without Inducing Apoptosis

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• 作者：Musarat Ishaq (1)
  Jiajie Hu (1)
  Xiaoyun Wu (1)
  Qiong Fu (1)
  Yalin Yang (1)
  Qingzhen Liu (1)
  Deyin Guo (1)
  
• 关键词：HIV ; 1 ; RNA helicase DDX3 ; RNAi ; Short hairpin RNA ; RNA nuclear export
• 刊名：Molecular Biotechnology
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：39
• 期：3
• 页码：231-238
• 全文大小：326KB
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• 作者单位：Musarat Ishaq (1)
  Jiajie Hu (1)
  Xiaoyun Wu (1)
  Qiong Fu (1)
  Yalin Yang (1)
  Qingzhen Liu (1)
  Deyin Guo (1)
  
  1. State Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China
  

文摘

The targeting of a cellular co-factor, rather than the HIV-1-specific RNAs, by small interfering RNAs holds promise as the rapid mutational ability of the HIV-1 genome may obviate the potential clinical use of RNAi against this virus. The DEAD-box RNA helicase DDX3 is an essential Rev co-factor in the CRM1-Rev-RRE complex that promotes the export of unspliced and single-spliced HIV-1 RNAs from the nucleus to cytoplasm. In this report, human DDX3 was targeted by specific short hairpin RNAs, and the down-regulation of cell's endogenous DDX3 suppressed the nuclear export of unspliced HIV-1 RNAs but did not affect the cell viability. We further showed that the knockdown of cellular DDX3 could effectively inhibit the replication of HIV-1. Therefore, the current results suggest that the RNA helicase DDX3 may become a potential target by RNAi for future genetic therapy of HIV/AIDS.