Identification of aldehyde reductase catalyzing the terminal step for conversion of xylose to butanetriol in engineered Escherichia coli

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• 作者：Kris Ni?o G. Valdehuesa ; Won-Keun Lee…
• 关键词：1 ; 2 ; 4 ; butanetriol ; Escherichia coli ; YqhD ; Aldehyde reductase ; Xylose ; Dahms pathway
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：38
• 期：9
• 页码：1761-1772
• 全文大小：1,707 KB
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• 作者单位：Kris Ni?o G. Valdehuesa (1)
  Won-Keun Lee (2)
  Kristine Rose M. Ramos (1)
  Rhudith B. Cabulong (1)
  JiSoo Choi (1)
  Huaiwei Liu (1) (3)
  Grace M. Nisola (1)
  Wook-Jin Chung (1)
  
  1. Department of Energy Science and Technology (DEST), Energy and Environment Fusion Technology Center (E2FTC), Myongji University, Yongin City, Gyeonggi-do, Republic of Korea
  2. Division of Bioscience and Bioinformatics, Myongji University, Yongin City, Gyeonggi-do, Republic of Korea
  3. Bioengineering Department, University of Illinois-Urbana Champaign, Urbana, IL, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

Biosynthetic pathways for the production of biofuels often rely on inherent aldehyde reductases (ALRs) of the microbial host. These native ALRs play vital roles in the success of the microbial production of 1,3-propanediol, 1,4-butanediol, and isobutanol. In the present study, the main ALR for 1,2,4-butanetriol (BT) production in Escherichia coli was identified. Results of real-time PCR analysis for ALRs in EWBT305 revealed the increased expression of adhP, fucO, adhE, and yqhD genes during BT production. The highest increase of expression was observed up to four times in yqhD. Singular deletion of adhP, fucO, or adhE gene showed marginal differences in BT production compared to that of the parent strain, EWBT305. Remarkably, yqhD gene deletion (KBTA4 strain) almost completely abolished BT production while its re-introduction (wild-type gene with its native promoter) on a low copy plasmid restored 75?% of BT production (KBTA4-2 strain). This suggests that yqhD gene is the main ALR of the BT pathway. In addition, KBTA4 showed almost no NADPH-dependent ALR activity, but was also restored upon re-introduction of the yqhD gene (KBTA4-2 strain). Therefore, the required ALR activity to complete the BT pathway was mainly contributed by YqhD. Increased gene expression and promiscuity of YqhD were both found essential factors to render YqhD as the key ALR for the BT pathway.