Growth and biochemical analysis of evergreen haloxeric tree species Salvadora oleoides and Salvadora persica under NaCl stress

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• 作者：Juleri M. Upendra ; Harchand R. Dagla
• 关键词：Salvadora ; Arid environment ; Peroxidase ; Jaal ; NaCl tolerance
• 刊名：Acta Physiologiae Plantarum
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：38
• 期：5
• 全文大小：2,427 KB
• 参考文献：Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107:1049–1054PubMed PubMedCentral
  Belcarz A, Ginalska G, Kowalewska B, Kulesza P (2007) Spring cabbage peroxidases potential tool in biocatalysis and bioelectrocatalysis. Photochemistry. doi:10.​1016/​j.​phytochem.​08.​018
  Bohnert HJ, Gong Q, Li P, Ma S (2006) Unraveling abiotic stress tolerance mechanisms—getting genomics going. Curr Opin Plant Biol 9:180–188CrossRef PubMed
  Bradford MM (1976) A rapid and sensitive for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRef PubMed
  Carretero CL, Cantos M, García JL, Troncoso A (2007) In vitro–ex vitro salt (NaCl) tolerance of cassava (Manihot esculenta Crantz) plants. In Vitro Cell Dev Biol-Plant 43:364–369CrossRef
  Christensen JH, Bauw G, Welinder GK, Montagu MV, Boerjan W (1998) Purification and characterization of peroxidases correlated with lignification in poplar xylem. Plant Physiol 118:125–135CrossRef PubMed PubMedCentral
  Dagla HR, Shekhawat NS (2005a) Plant regeneration from tissue culture of Chloris virgata: a salt-tolerant desert grass. Indian J. Biotechnol 4:400–403
  Dagla HR, Shekhawat NS (2005b) In vitro multiplication of Haloxylon recurvum (Moq)—a plant for saline soil reclamation. J Plant biotech. 7(3):1–5
  Dagla HR, Shekhawat NS (2006) Little known use of Haloxylon spp. in traditional food. Nat Prod Rad 5(2):131–132
  Dagla HR, Paliwal A, Shekhawat NS (2007) Oran: a scared way for biodiversity conservation in Indian Thar Desert. Curr Sci 93(3):279
  Dagla HR, Paliwal A, Rathore MS, Shekhawat NS (2012) Micropropagation of Leptadenia pyrotechnica (Forsk.) Decne: a multipurpose plant of an arid environment. J Sustain Forest 31:283–293CrossRef
  Dagla HR, Nair S, Vyas DK, Upendra JM (2014) In vitro culture of plants from arid environments. In: Tuteja N, Gill SS (eds) Climate change and abiotic stress tolerance. WILEY-VHC Verlag GmbH & Co.KGaA, pp 933–938
  FAO (1993) Conservation of genetic resources in tropical forest management: Principles and concepts. FAO Forestry Paper 107. FAO, Rome
  Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytol 179:945–963CrossRef PubMed
  Gehlot HS, Tak N, Dagla HR, Davis TD (2014) Indigenous and modern scientific strategies for characterization, conservation and sustainable utilization of bio-resources of the Indian Thar Desert. J Arid Land Stud 24–1:5–8
  Glenn EP, Brown JJ, Blumwald E (1999) Salt tolerance and crop potential of halophytes. Crit Rev Plant Sci 18:227–255CrossRef
  Hamed KB, Ellouzi H, Talbi OZ, Hessini K, Slama I, Ghnaya T, Bosch SM, Savoure A, Abdelly C (2013) Physiological response of halophytes to multiple stresses. Funct Plant Biol 40(9):883–896
  Heuzé V, Tran G, Giger-Reverdin S (2013) Saltbush (Salvadora persica). Feedipedia.org. A programme by INRA, CIRAD, AFZ and FAO. http://​www.​feedipedia.​org/​node/​97
  Karp AS, Kresovich KV, Bhat WG, Ayad Hodgkin T (1997) Molecular tools in plant genetic resources conservation: a guide to the technologies. IPGRI Technical Bulletin No. 2. International Plant Genetic Resources Institute, Rome, Italy
  Lee BR, Kim KY, Jung WJ, Avice JC, Ourry A, Kim TH (2007) Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.). J Exp Bot 58(6):1271–1279CrossRef PubMed
  Marjamaa K, Kukkola EM, Fagerstedt KV (2009) The role of xylem class III peroxidases in lignifications. J Exp Bot 60(2):367–376CrossRef PubMed
  Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRef
  Sha Valli Khan PS, Hoffmann L, Renaut J, Hausman JF (2007) Current initiatives in proteomics for the analysis of plant salt tolerance. Curr Sci 93(6):807–817
  Shabala S (2013) Learning from halophytes: physiological basis and strategies to improve abiotic stress tolerance in crops. Ann Bot 112(7):1209–1221CrossRef PubMed PubMedCentral
  Sharma V, Ramawat KG (2013) Salinity-induced modulation of growth and antioxidant activity in the callus cultures of miswak (Salvadora persica). 3 Biotech 3(1):11–17CrossRef PubMedCentral
  Worthington Enzyme Manual (1972) Enzymes, enzyme reagents, related biochemicals. Worthington Biochemical Corporation, Freehold, New Jersey, USA. p. 216
  Yadav JP, Saini S, Kalia AN, Dangi AS (2008) Hypoglycemic and hypolipidemic activity of ethanolic extract of Salvadora oleoides in normal and alloxan-induced diabetic rats. Indian J Pharmacol 40(1):23–27CrossRef PubMed PubMedCentral
  
• 作者单位：Juleri M. Upendra (1)
  Harchand R. Dagla (1)
  
  1. Plant Biotechnology and Molecular Biology Laboratory, Department of Botany, J. N. Vyas University, Jodhpur, India
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

In vitro growth, development, total soluble proteins and peroxidase profiles of Salvadora oleoides and Salvadora persica under NaCl stress were analysed in the present investigation. The plants are evergreen haloxeric tree species of family Salvadoraceae. Shoot apex from natural plants were initially used for screening of NaCl tolerance on MS culture medium. Shoot apex of S. oleoides and S. persica could survive optimally up to 200 and 100 mM NaCl. Axillary buds from nodal shoot segments of S. oleoides and S. persica were activated on 6 and 4 μM BAP, and were used further for extraction of total soluble proteins and peroxidases. Total soluble proteins were increased up to 150 mM NaCl in S. oleoides, but decline above 50 mM NaCl in S. persica. Peroxidase activity remained almost constant in S. oleoides at all the concentrations and duration of NaCl, but increased at 100 mM NaCl during fourth week of treatment in S. persica. Eleven peroxidase isozymes were observed in zymogram of S. oleoides. Isozymes P1, P2, P3, and P4 were slightly appeared, but P6 isozyme was lacking in S. persica. The P5 isozyme was more prominent in S. persica than S. oleoides. Isozyme P9 of S. persica was visible during the first week of NaCl treatment, but disappeared in the fourth week. Molecular biology of these plants can be useful further for the understanding of stress tolerance mechanisms for prospects.