Alleviation of ultraviolet-C-induced oxidative damage through overexpression of cytosolic ascorbate peroxidase

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• 作者：Saurabh C. Saxena (1)
  Pankaj K. Joshi (1)
  Bernhard Grimm (2)
  Sandeep Arora (3)
  
• 关键词：ascorbate peroxidase ; Nicotiana tabacum ; UV ; C radiation ; oxidative stress
• 刊名：Biologia
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：66
• 期：6
• 页码：1052-1059
• 全文大小：240KB
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• 作者单位：Saurabh C. Saxena (1)
  Pankaj K. Joshi (1)
  Bernhard Grimm (2)
  Sandeep Arora (3)
  
  1. Department of Biochemistry, GB Pant University of Agriculture & Technology, Pantnagar, 263145, Uttarakhand, India
  2. Department of Plant Physiology, Institute of Biology, Humboldt University, Berlin, Germany
  3. Department of Molecular Biology & Genetic Engineering, GB Pant University of Agriculture & Technology, Pantnagar, 263145, Uttarakhand, India
  

文摘

Experiments were conducted to investigate the relationship between ultraviolet (UV) C-induced oxidative damage and the activity of ascorbate peroxidase (APX), using transgenic tobacco (Nicotiana tabacum L. cv. Petit Havana) plants overexpressing cytosolic APX gene (apx1). Transgenic plants having 2.3 fold higher total APX activity, as compared to the wild type plants, showed normal morphological characters. Exposure of 70-day-old plants to fixed intensity UV-C radiation caused an increase in the malondialdehyde (MDA) content in wild type as well as transgenic plants. However, the wild type plants showed significantly higher (p < 0.05) lipid peroxidation as compared to the transgenic plants. Higher proline accumulation was recorded in transgenic plants as compared to the wild type plants, after 24 hours of UV-C exposure. Although the ascorbate content decreased continuously with increasing exposure to UV-C radiation, yet the wild type plants exhibited higher ascorbate levels than the transgenic plants. A marked difference in H2O2 content, between the wild type and transgenic plants, was consistently observed up to 20 hours of UV-C exposure. A direct correlation of ascorbate, MDA and H2O2 levels was recorded with the extent of oxidative stress, signifying that these could be used as potential bio-marker molecules for oxidative stress. The results clearly demonstrate that overexpression of cytosolic APX can protect tobacco plants from UV-C-induced oxidative damage.