GA-20 oxidase as a candidate for the semidwarf gene sdw1/denso in barley

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• 作者：Qiaojun Jia ; Jingjuan Zhang ; Sharon Westcott ; Xiao-Qi Zhang ; Mathew Bellgard ; Reg Lance and Chengdao Li
• 关键词：Semidwarf ; Comparative mapping ; Candidate gene ; Gibberellic acid ; SNP ; Plant height ; sdw1/denso
• 刊名：Functional & Integrative Genomics
• 出版年：2009
• 出版时间：May, 2009
• 年：2009
• 卷：9
• 期：2
• 页码：255-262
• 全文大小：254.6 KB

文摘

The barley sdw1/denso gene not only controls plant height but also yield and quality. The sdw1/denso gene was mapped to the long arm of chromosome 3H. Comparative genomic analysis revealed that the sdw1/denso gene was located in the syntenic region of the rice semidwarf gene sd1 on chromosome 1. The sd1 gene encodes a gibberellic acid (GA)-20 oxidase enzyme. The gene ortholog of rice sd1 was isolated from barley using polymerase chain reaction. The barley and rice genes showed a similar gene structure consisting of three exons and two introns. Both genes share 88.3 % genomic sequence similarity and 89 % amino acid sequence identity. A single nucleotide polymorphism was identified in intron 2 between barley varieties Baudin and AC Metcalfe with Baudin known to contain the denso semidwarf gene. The single nucleotide polymorphism (SNP) marker was mapped to chromosome 3H in a doubled haploid population of Baudin ¡Á AC Metcalfe with 178 DH lines. Quantitative trait locus analysis revealed that plant height cosegregated with the SNP. The sdw1/denso gene in barley is the most likely ortholog of the sd1 in rice. The result will facilitate understanding of the molecular mechanism controlling semidwarf phenotype and provide a diagnostic marker for selection of semidwarf gene in barley.