Lake Kinneret phytoplankton: integrating classical and molecular taxonomy

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• 作者：Ruth N. Kaplan-Levy ; Alla Alster-Gloukhovski ; Yael Benyamini ; Tamar Zohary
• 关键词：Barcode chlorophyta ; Cyanobacteria ; Online catalog ; rbcL ; SSU ; ITS ; Taxonomy
• 刊名：Hydrobiologia
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：764
• 期：1
• 页码：283-302
• 全文大小：1,681 KB
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• 作者单位：Ruth N. Kaplan-Levy (1)
  Alla Alster-Gloukhovski (1)
  Yael Benyamini (1)
  Tamar Zohary (1)
  
  1. Yigal Allon Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, P.O. Box 447, 14950, Migdal, Israel
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5117

文摘

The Kinneret phytoplankton biodiversity has been monitored on a regular basis since 1969, with the taxonomic information stored as a digital online catalog (http://鈥媖inneret.鈥媜cean.鈥媜rg.鈥媔l/鈥媝hyt_鈥媍at_鈥媗istView.鈥媋spx) containing photographs and morphological descriptions. Our aim was to upgrade this ID tool by adding to it a consensus DNA sequence as a species identifier鈥擠NA barcode. A two-gene system approach was applied, sequencing DNA fragments from the Ribulose-1,5-bisphosphate carboxylase oxygenase large subunit (rbcL) and the rRNA small subunit and intragenic space (SSU-ITS). Today, 72 species isolated from Lake Kinneret, are at different stages of processing. The barcoding process was completed for 26 of those. Barcoding has enabled us to support current nomenclature, clarify the taxonomic names of species whose identification was previously inconclusive, classify to species level taxa previously identified to genus only, discover species new to the lake, and update current nomenclature. The construction of the DNA barcode database stands as the first brick of knowledge upon which further experimental work can be conducted, enabling fast and accurate identification of the Kinneret phytoplankton. Keywords Barcode chlorophyta Cyanobacteria Online catalog rbcL SSU-ITS Taxonomy