Identification of genetic/epigenetic biomarkers for supporting decision of VOCs exposure

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• 作者：Yu Ri An (17101)
  Seung Jun Kim (27101)
  So-Yeon Yu (17101) (27101)
  Hyo-Jung Yoon (27101)
  Mi-Kyung Song (37101)
  Jae-Chun Ryu (37101)
  Seung Yong Hwang (17101) (27101)
  
• 关键词：Volatile organic compounds ; Biomarker ; Epigenetic ; MicroRNA ; Methylation
• 刊名：BioChip Journal
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：7
• 期：1
• 页码：1-5
• 全文大小：332 KB
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• 作者单位：Yu Ri An (17101)
  Seung Jun Kim (27101)
  So-Yeon Yu (17101) (27101)
  Hyo-Jung Yoon (27101)
  Mi-Kyung Song (37101)
  Jae-Chun Ryu (37101)
  Seung Yong Hwang (17101) (27101)
  
  17101. Department of Biochemistry, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Korea
  27101. GenoCheck Co. Ltd., Sangnok-gu, Ansan, Gyeonggi-do, Korea
  37101. Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology, P.O. Box 131, Cheongryang, Seoul, 130-650, Korea
  
• ISSN：2092-7843

文摘

Ethylbenzene, toluene and xylene are widely used volatile organic compounds (VOCs). VOCs are dangerous to human health or cause harm to the environment. The assessment of VOC exposure maybe carried out through a number of techniques. One of them, the epigenetic biomarker applies a new technology. Environmental epigenetics focuses on how cells or organisms respond to environmental factors to create altered phenotypes or diseases. In this study, our intention was to conduct an investigation of epigenetic biomarkers in VOCs. For the experiment, we used the DNAs and RNAs from VOC exposed human blood and VOCs treated cell lines. Then, we compared the data using microarray fold change, real-time PCR and methylation specific PCR. As a result, we identified five microRNAs (miR-520g, miR-424, miR-210, miR-21, and miR-142-3p), three methylation regions (SERPINB5, ZC3H3, and PCSK6) and four transcripts (CRCT1, RUNX3, PCDH11X, and PCSK6), which could be used as biomarkers for VOCs, becommings the foundation for a system which can predict VOC exposure.