External carbonic anhydrase in three Caribbean corals: quantification of activity and role in CO2 uptake

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• 作者：Anna L. Tansik ; William K. Fitt ; Brian M. Hopkinson
• 关键词：Coral ; Carbonic anhydrase ; Photosynthesis ; CO2
• 刊名：Coral Reefs
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：34
• 期：3
• 页码：703-713
• 全文大小：790 KB
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• 作者单位：Anna L. Tansik (1)
  William K. Fitt (2)
  Brian M. Hopkinson (1)
  
  1. Department of Marine Sciences, University of Georgia, Athens, GA, 30602, USA
  2. School of Ecology, University of Georgia, Athens, GA, 30602, USA
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Earth sciences
  Oceanography
  Geology
  Sedimentology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0975

文摘

Scleractinian corals have complicated inorganic carbon (C i) transport pathways to support both photosynthesis, by their symbiotic dinoflagellates, and calcification. The first step in C i acquisition, uptake into the coral, is critical as the diffusive boundary layer limits the supply of CO2 to the surface and HCO3 ?/sup> uptake is energy intensive. An external carbonic anhydrase (eCA) on the oral surface of corals is thought to facilitate CO2 uptake by converting HCO3 ?/sup> into CO2, helping to overcome the limitation imposed by the boundary layer. However, this enzyme has not yet been identified or detected in corals, nor has its activity been quantified. We have developed a method to quantify eCA activity using a reaction–diffusion model to analyze data on 18O removal from labeled C i. Applying this technique to three species of Caribbean corals (Orbicella faveolata, Porites astreoides, and Siderastrea radians) showed that all species have eCA and that the potential rates of CO2 generation by eCA greatly exceed photosynthetic rates. This demonstrates that eCA activity is sufficient to support its hypothesized role in CO2 supply. Inhibition of eCA severely reduces net photosynthesis in all species (on average by 46?±?27?%), implying that CO2 generated by eCA is a major carbon source for photosynthesis. Because of the high permeability of membranes to CO2, CO2 uptake is likely driven by a concentration gradient across the cytoplasmic membrane. The ubiquity of eCA in corals from diverse genera and environments suggests that it is fundamental for photosynthetic CO2 supply.