Isolation of endophytic bacteria from embryogenic suspension culture of banana and assessment of their plant growth promoting properties

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• 作者：John C. Jimtha (1)
  P. V. Smitha (2)
  C. Anisha (1)
  Thomas Deepthi (3)
  George Meekha (3)
  E. K. Radhakrishnan (1)
  G. P. Gayatri (2)
  A. Remakanthan (2)
  
• 关键词：Endophytic bacteria ; Indole acetic acid ; Plant growth promotion (PGP) ; Viable ; but ; non ; culturable (VBNC) ; Musa sp. ; Plant tissue culture
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：118
• 期：1
• 页码：57-66
• 全文大小：
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• 作者单位：John C. Jimtha (1)
  P. V. Smitha (2)
  C. Anisha (1)
  Thomas Deepthi (3)
  George Meekha (3)
  E. K. Radhakrishnan (1)
  G. P. Gayatri (2)
  A. Remakanthan (2)
  
  1. School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, 686 560, Kerala, India
  2. Department of Botany, Sree Neelakanta Government Sankrit College, Pattambi, 679 306, Kerala, India
  3. Indian Institute of Science Education and Research, Kolkata, Mohanpur Campus, Nadia District, Mohanpur, 741 252, West Bengal, India
  
• ISSN：1573-5044

文摘

Endophytic microorganisms have been reported from various plants. In the current study, somatic embryogenic cultures of banana (Musa accuminata AAA cv. Grand Naine) were found to have association with endophytic bacteria which were present initially in a covert state. The presence of bacteria was detected only in suspension cultures derived from the somatic embryogenic cultures. The bacteria isolated from embryogenic cell suspension culture were identified as Ralstonia sp. and Bacillus sp. The Ralstonia sp. interestingly showed the presence of various plant growth promoting properties including indole acetic acid and siderophore production. Also the strain was found to have the potential to solubilize phosphate and produce ammonia. Growth enhancement effect of Ralstonia sp. on Vigna radiata seedlings showed promising results and the growth parameters were found to be statistically significant when compared to control. Identification and confirmation of the plant growth promoting properties of Ralstonia sp. makes the study significant with promising applications.