Ultra high performance liquid chromatography–high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets

详细信息    查看全文

• 作者：Amanda J. Lloyd ; Manfred Beckmann ; Thomas Wilson ; Kathleen Tailliart…
• 关键词：Metabolomics ; Lipidomics ; Plasma ; Canine breeds ; Accurate mass metabolomic profiling
• 刊名：Metabolomics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：13
• 期：2
• 全文大小：824KB
• 刊物主题：Biochemistry, general; Molecular Medicine; Cell Biology; Developmental Biology; Biomedicine, general;
• 出版者：Springer US
• ISSN：1573-3890
• 卷排序：13

文摘

Introduction and objectivesThe purpose of this study was to use high accurate mass metabolomic profiling to investigate differences within a phenotypically diverse canine population, with breed-related morphological, physiological and behavioural differences. Previously, using a broad metabolite fingerprinting approach, lipids appear to dominate inter- and intra- breed discrimination. The purpose here was to use Ultra High Performance Liquid Chromatography–High Resolution Mass Spectrometry (UHPLC–HRMS) to identify in more detail, inter-breed signatures in plasma lipidomic profiles of home-based, client-owned dogs maintained on different diets and fed according to their owners’ feeding regimens.