Seasonal changes in isoform composition of giant proteins of thick and thin filaments and titin (connectin) phosphorylation level in striated muscles of bears (Ursidae, Mammalia)

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• 作者：N. N. Salmov ; I. M. Vikhlyantsev ; A. D. Ulanova ; Yu. V. Gritsyna…
• 关键词：hibernation ; brown bear ; Himalayan black bear ; striated muscles ; thick (myosin) filaments ; thin (actin) filaments ; titin ; nebulin ; isoforms of myosin heavy chains ; Pro ; Q Diamond
• 刊名：Biochemistry (Moscow)
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：80
• 期：3
• 页码：343-355
• 全文大小：1,517 KB
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  6. Tinker, D B, Harlow, H J, Beck, T D (1998) Protein use and muscle-fiber changes in free-ranging, hibernating black bears. Physiol. Zool. 71: pp. 414-424 CrossRef
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  9. Hershey, J D, Robbins, C T, Nelson, O L, Lin, D C (2008) Minimal seasonal alterations in the skeletal muscle of captive brown bears. Physiol. Biochem. Zool. 81: pp. 138-147 CrossRef
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  11. Barrows, N D, Nelson, O L, Robbins, C T, Rourke, B C (2011) Increased cardiac alpha-myosin heavy chain in left atria and decreased myocardial insulin-like growth factor (Igf-I) expression accompany low heart rate in hibernating grizzly bears. Physiol. Biochem. Zool. 84: pp. 1-17 CrossRef
  12. Tatsumi, R, Hattori, A (1995) Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose. Anal. Biochem. 224: pp. 28-31 CrossRef
  13. Vikhlyantsev, I M, Podlubnaya, Z A, Kozlovskaya, I B (2004) New titin isoforms in skeletal muscles of mammals. Dokl. Biochem. Biophys. 395: pp. 111-113 CrossRef
  14. Vikhlyantsev, I M, Podlubnaya, Z A (2006) On the problem of titin isoforms. Biofizika 51: pp. 951-958
  15. Tikunov, B A, Sweeney, H L, Rome, L C (2001) Quantitative electrophoretic analysis of myosin heavy chains in single muscle fibers. J. Appl. Physiol. 90: pp. 1927-1935
  16. Borbely, A, Falcao-Pires, I, Heerebeek, L, Hamdani, N, Edes, I, Gavina, C, Leite-Moreira, A F, Bronzwaer, J G, Papp, Z, Velden, J, Stienen, G J, Paulus, W J (2009) Hypophosphorylation of the Stiff N2B titin isoform raises cardiomyocyte resting tension in failing human myocardium. Circ. Res. 104: pp. 780-786 CrossRef
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  18. Vikhlyantsev, I M, Okuneva, A D, Shpagina, M D, Shumilina, Yu V, Molochkov, N V, Salmov, N N, Podlubnaya, Z A (2011) Changes in isoform composition, structure and functional properties of titin from the myocardium of Mongolian gerbil (Meriones unguiculatus) after exposure to real microgravity. Biochemistry (Moscow) 76: pp. 1312-1
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Microbiology
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3040

文摘

Seasonal changes in the isoform composition of thick and thin filament proteins (titin, myosin heavy chains (MyHCs), nebulin), as well as in the phosphorylation level of titin in striated muscles of brown bear (Ursus arctos) and hibernating Himalayan black bear (Ursus thibetanus ussuricus) were studied. We found that the changes that lead to skeletal muscle atrophy in bears during hibernation are not accompanied by a decrease in the content of nebulin and intact titin-1 (T1) isoforms. However, a decrease (2.1-.4-fold) in the content of T2 fragments of titin was observed in bear skeletal muscles (m. gastrocnemius, m. longissimus dorsi, m. biceps) during hibernation. The content of the stiffer N2B titin isoform was observed to increase relative to the content of its more compliant N2BA isoform in the left ventricles of hibernating bears. At the same time, in spite of the absence of decrease in the total content of T1 in the myocardium of hibernating brown bear, the content of T2 fragments decreased ?.6-fold. The level of titin phosphorylation only slightly increased in the cardiac muscle of hibernating brown bear. In the skeletal muscles of brown bear, the level of titin phosphorylation did not vary between seasons. However, changes in the composition of MyHCs aimed at increasing the content of slow (I) and decreasing the content of fast (IIa) isoforms of this protein during hibernation of brown bear were detected. Content of MyHCs I and IIa in the skeletal muscles of hibernating Himalayan black bear corresponded to that in the skeletal muscles of hibernating brown bear.