Distinguishing feral and managed honeybees (Apis mellifera) using stable carbon isotopes

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• 作者：Lucy M. Anderson (1)
  Travis M. Dynes (1)
  Jennifer A. Berry (2)
  Keith S. Delaplane (2)
  Lydia L. McCormick (1)
  Berry J. Brosi (1)
  
• 关键词：feral honeybees ; stable isotopes ; carbon ; photosynthesis pathways ; isotopic fractionation
• 刊名：Apidologie
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：45
• 期：6
• 页码：653-663
• 全文大小：714 KB
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• 作者单位：Lucy M. Anderson (1)
  Travis M. Dynes (1)
  Jennifer A. Berry (2)
  Keith S. Delaplane (2)
  Lydia L. McCormick (1)
  Berry J. Brosi (1)
  
  1. Department of Environmental Studies, Emory University, 400 Dowman Drive, Suite E510, Atlanta, GA, 30322, USA
  2. Department of Entomology, University of Georgia, Athens, GA, 30602, USA
  
• ISSN：1297-9678

文摘

The ability to distinguish feral and managed honeybees (Apis mellifera) has applications in studies of population genetics, parasite transmission, pollination, interspecific interactions, and bee breeding. We evaluated a diagnostic test based on theoretical differences in stable carbon isotope ratios generated by supplemental feeding. We evaluated (1) if carbon isotope ratios can distinguish feral and managed honeybees and (2) the temporal persistence of the signal after discontinuation of supplemental feeding. We compared carbon isotope ratios from four types of experimental colonies: feral, managed with and without supplemental feed, and managed with 13C-labeled glucose added to supplemental feed. There was a significant difference between the isotopic signatures of colonies receiving supplemental feed and unfed feral colonies. This difference, however, only persisted for a few weeks after supplemental feeding was discontinued, suggesting that this method may work best under a narrow range of conditions. This work highlights the potential for exploiting temporal turnover of carbon in bee tissues as a tool for studying nutrient flow in honeybee colonies.