Rapid detection of synthetic biomarkers of Escherichia coli in water using microAnalyzer: a field dependence study

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• 作者：Lena Ganda Saptalena (1) lena.saptalena@uni-due.de
  Klaus Kerpen (1)
  Andriy Kuklya (1)
  Ursula Telgheder (1)
• 关键词：microAnalyzer – ; Escherichia coli – ; Differential mobility spectrometry – ; Field dependence study – ; Rapid detection
• 刊名：International Journal for Ion Mobility Spectrometry
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：15
• 期：2
• 页码：47-53
• 全文大小：732.3 KB
• 参考文献：1. European Centre for Disease Prevention and Control (ECDC) (2011) Rapid risk assessment update: Outbreak of Shiga toxin-producing E. coli (STEC) O104:H4 2011 in the EU, 8 July 2011. European Centre for Disease Prevention and Control, Technical Report – July 12, 2011. http://ecdc.europa.eu/en/publications/Publications/110712_TER_Risk_Assessement_Ecoli.pdf. Accessed 11 Aug 2011
  2. European Food Safety Authority (EFSA) (2011) Tracing seeds, in particular fenugreek (Trigonella foenum-graecum) seeds, in relation to the Shiga toxin-producing E. coli (STEC) O104:H4 2011 Outbreaks in Germany and France. European Food Safety Authority, Technical Report – July 5, 2011. http://www.efsa.europa.eu/en/supporting/doc/176e.pdf. Accessed 11 Aug 2011
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• 作者单位：1. Faculty of Chemistry, Instrumental Analytical Chemistry, University of Duisburg-Essen, Universit盲tsstr. 5, 45141 Essen, Germany
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Physical Chemistry
  Molecular Medicine
  Proteomics
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Safety in Chemistry and Dangerous Goods
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1865-4584

文摘

Mixtures of 12 known volatile metabolites of Escherichia coli (2,5-dimethyltetrahydrofuran, dimethyl disulfide, 2-heptanone, 2,5-dimethylpyrazine, benzaldehyde, dimethyl trisulfide, 2-nonanone, nonanal, decanal, 2-undecanone, indole, and 2-tridecanone) were prepared from internal standards in water. The headspace gases were then analyzed using microAnalyzer™, a miniaturized Gas Chromatography-Differential Mobility Spectrometry (GC-DMS) system. Differential mobility spectra showed monomer peaks of the analytes with the observed retention times below 3 min. Dispersion voltages, determining the dependence of signal intensities and compensation voltages of each analyte on the applied radio frequency (RF) voltages, were recorded for positive and negative modes. It was found that at increasing RF voltages the monomer peaks of compounds of lower molecular masses tend to shift to more negative compensation voltages; whereas, those of higher molecular masses tend to shift to more positive compensation voltages. The shift results in increase separation among each peak. The signal intensities for all peaks, however, tend to decrease at higher RF voltages. The RF voltage which gave optimum peaks separation and signal resolution was 1,200 V (corresponds to 24 kV/cm). This study demonstrates the potential of microAnalyzer™ as an integrated gas chromatograph detector for a preliminary screening system in the on-site detection of E. coli in water. The operational principle of the instrument is also presented in this study.