Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solim?es Region, Ama

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• 作者：Antonio Marques Pereira Júnior ; Carolina Bioni Garcia Teles…
• 关键词：Diversity ; Amazon environments ; Cutaneous Leishmaniasis ; Richness ; Diversity ; Vectors
• 刊名：Parasites & Vectors
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：8
• 期：1
• 全文大小：3,807 KB
• 参考文献：1. Ready, PD (2013) Biology of phlebotomine sand flies as vectors of disease agents. Annu Rev Entomol 58: pp. 227-250 CrossRef
  2. Sinan. Sistema de informa??es de agravos de notifica??o. 2014. [http://dtr2004.saude.gov.br/sinanweb]. Assessed 15 Feb 2014.
  3. Camara Coelho, LI, Paes, M, Guerra, JAO, Barbosa, MG, Coelho, C, Lima, B (2011) Characterization of Leishmania spp. causing cutaneous leishmaniasis in Manaus, Amazonas, Brazil. Parasitol Res 108: pp. 671-677 CrossRef
  4. Dias-Lima, AG, Castellón, EG, Sherlock, I (2003) Flebotomíneos (Diptera: Psychodidae) de uma floresta primária de terra firme da Esta??o experimental de silvicultura tropical, estado do Amazonas, Brasil. Acta Amaz 33: pp. 303-316 CrossRef
  5. Pessoa, FAC, Medeiros, JF, Barrett, TV (2007) Effects of timber harvest on phlebotomine sand flies (Diptera: Psychodidae) in a production forest: abundance of species on tree trunks and prevalence of trypanosomatids. Mem Inst Oswaldo Cruz 102: pp. 593-599 CrossRef
  6. Rocha, LC, Freitas, RA, Franco, AMR (2013) Phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) in urban rainforest fragments, Manaus - Amazonas State, Brazil. Acta Trop 126: pp. 103-109 CrossRef
  7. Figueira, EAG, Silva, GS, Chagas, EC, Shimabukuro, PHF (2013) Phlebotomine sandflies (Diptera: Psychodidae) from Lábrea, state of Amazonas, Brazil, with a description of Evandromyia (Aldamyia) apurinan Shimabukuro, Figueira & Silva, sp. nov. Mem Inst Oswaldo Cruz 108: pp. 280-287 CrossRef
  8. Ramos, WR, Medeiros, JF, Juli?o, GR, Ríos-Velásquez, CM, Marialva, EF, Desmouliére, SJM (2014) Anthropic effects on sand fly (Diptera: Psychodidae) abundance and diversity in an Amazonian rural settlement, Brazil. Acta Trop 139: pp. 44-52 CrossRef
  9. Shimabukuro, PHF, Tolezano, JE, Galati, EAB (2011) Chave de Identifica??o Ilustrada dos Phlebotominae (Diptera, Psychodidae) do Estado de S?o Paulo, Brasil. Pap Avulsos Zool 51: pp. 399-441
  10. Alves, VR, Freitas, RA, Santos, FL, Oliveira, AFJ, Barrett, TV, Shimabukuro, PHF (2012) Sand flies (Diptera, Psychodidae, Phlebotominae) from Central Amazonia and four new records for the Amazonas state, Brazil. Rev Bras Entomol 56: pp. 220-227 CrossRef
  11. Oliveira, AFJ, Aguiar, NO, Freitas, RA, Pessoa, FAC (2013) New records of phlebotomine fauna (Diptera, Psychodidae) in the Aman? sustainable development reserve, Amazonas, Brazil. Uakari 9: pp. 55-59
  12. Ladeia-Andrade, S, Fe, NF, Sanguinette, CC, Andrade Filho, JD (2014) Description of Trichophoromyia uniniensis, a new phlebotomine species (Diptera: Psychodidae: Phlebotominae) of Amazonas State Brazil. Parasit Vectors 7: pp. 400 CrossRef
  13. Guerra, JAO, Talhari, S, Paes, MG, Garrido, M, Talhari, JM (2003) Aspectos clínicos e diagnósticos da leishmaniose tegumentar americana em militares simultaneamente expostos à infec??o na Amaz?nia. Rev Soc Bras Med Trop 36: pp. 587-590 CrossRef
  14. Guerra, JAO, Ribeiro, JAS, Coelho, LIARC, Barbosa, MGV, Paes, MG (2006) Epidemiologia da leishmaniose tegumentar na Comunidade S?o Jo?o, Manaus, Amazonas Brasil. Cad Saude Publica 22: pp. 2319-2327 CrossRef
  15. Naiff Júnior, RD, Pinheiro, FG, Naiff, MF, Souza, I, Castro, LM, Menezes, MP (2009) Estudo de uma série de casos de leishmaniose tegumentar americana no município de Rio Preto da Eva, Amazonas, Brasil. Rev Patol Trop 38: pp. 103-114 CrossRef
• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
• 出版者：BioMed Central
• ISSN：1756-3305

文摘

Background Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations. Methods Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing. Results In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 -58.26%) and Nyssomyia antunesi (661 -11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality. Conclusions The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA.