Identification of a highly successful cryopreservation method (droplet-vitrification) for petunia

详细信息    查看全文

• 作者：Jin-Mei Zhang ; Bin Huang ; Xiao-Ning Zhang…
• 关键词：Droplet ; vitrification ; Genetic stability ; One ; factor experiment ; Orthogonal array ; Petunia
• 刊名：In Vitro Cellular & Developmental Biology - Plant
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：51
• 期：4
• 页码：445-451
• 全文大小：463 KB
• 参考文献：Benson EE (2008) Cryopreservation theory. In: Reed BM (ed) Plant cryopreservation: a practical guide. Springer, New York, pp 15-0View Article
  Berke MJ (1986) The glass state and survival of anhydrous biological systems. In: Leopold AC (ed) Membrane, metabolism and dry organisms. Cornell Univ. Press, Ithaca, pp 358-64
  Cellarova E, Skyba M, Urbanova M, Kapchina-Toteva V, Kosuth J, Harding K (2010) Physiological, biochemical and molecular characteristics of cryopreserved Hypericum perforatum L. shoot Tips. CryoLetters 31:249-60PubMed
  Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 47:5-6View Article
  Engelmann F, Sakai A (2007) Vitrification, encapsulation-vitrification and droplet-vitrification: a review. CryoLetters 28:151-72PubMed
  Fei YL, Liu QL, Ge H (2008) Chinese crops and their wild relatives-flower. China Agriculture Press, Beijing, pp 505-08
  Kong D, Shang H, Guo K, Liu Y, Zhang J, Wei H (2012) A study on optimizing the cryopreservation methods for Bama miniature pig semen. Exp Anim 61(5):533-42View Article PubMed
  Kriedt RA, Ramos-Fregonezi AMC, Beheregaray LB, Bonatto SL, Freitas LB (2011) Isolation, characterization, and cross-amplification of microsatellite markers for the Petunia integrifolia (Solanaceae) complex. Am Bot 98:e277–e279View Article
  Kuriyama A, Watanabe K, Ueno S (1989) Inhibitory effect of ammoniumion on recovery of cryopreserved rice cells. Plant Sci 64:231-35View Article
  Lyu SR, Wu WT, Hou CC, Hsieh WH (2010) Study of cryopreservation of articular chondrocytes using the Taguchi method. Cryobiology 60:165-76View Article PubMed
  Mandal BB, Ahuja-Ghosh S, Srivastava PS (2008) Cryopreservation of Dioscorea rotundata poir.: a comparative study with two cryogenic procedures and assessment of true-to-type of regenerants by RAPD analysis. CryoLetters 29:399-08PubMed
  Marco-Medina A, Luis Casas J, Swennen R, Panis B (2010) Cryopreservation of Thymus moroderi by droplet vitrification. CryoLetters 31:14-3PubMed
  Matsumoto T, Sakai A, Yamada K (1994) Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration. Plant Cell Rep 13:442-46View Article PubMed
  Matsumoto T, Akihiro T, Maki S, Mochida K, Kitagawa M, Tanaka D, Yamamoto S, Niino T (2013) Genetic stability assessment of wasabi plants regenerated from long-term cryopreserved shoot tips using morphological, biochemical and molecular analysis. CryoLetters 34:128-36PubMed
  McLellan MR (1995) Methods in molecular biology, vol. 38. In: Cryopreservation and freeze-drying protocols. Humana Press, Totowa, NJ, pp 133-144
  Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473-97View Article
  Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321-325PubMed Central View Article PubMed
  Nadarajan J, Staines HJ, Benson EE, Marzalina M, Krishnapillay B, Harding K (2006) Optimization of cryopreservation protocol for Sterculia cordata zygotic embryos using Taguchi experiments. J Trop For Sci 18:222-30
  Pinker I, Halmagyi A, Olbricht K (2009) Effects of sucrose preculture on cryopreservation by droplet-vitrification of strawberry cultivars and morphological stability of cryopreserved plants. CryoLetters 30:202-11PubMed
  Reed BM (2008) Plant cryopreservation: a practical guide. Springer, New York, pp 1-13View Article
  Reed BM, Castillo NRF, Bassil NV, Wada S (2010) Genetic stability of cryopreserved shoot tips of Rubus germplasm. In Vitro Cell Dev Biol Plant 46:246-56View Article
  Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30-3View Article PubMed
  Sekizawa K, Yamamoto SI, Rafique T, Fukui K, Niino T (2011) Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates. Plant Biotechnol 28:401-05View Article
  Teixeira AS, Gonzalez-Benito ME, Molina-Garcia AD (2013) Glassy state and cryopreservation of mint shoot tips. Biotechnol Prog 29:707-17View Article PubMed
  Vallejo-Marin M, Solis-Montero L, Bacies CFE, Lepais O (2011) Thirteen micro-satellites developed by SSR-enriched pyrosequencing for Solanum Rostratum (Slolanaceae) and related species. Am Bot 98:e296–e299View Article
  Volk GM, Bonnart R, Krueger R, Lee RR (2012) Cryopreservation of citrus shoot tips using micrografting for recovery. CryoLetters 33:418-26PubMed
  Zhang JM, Zhang XN, Lu XX, Xin X, Yin GK, He JJ, Xu YM, Chen XL (2014) Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructual studies on shoot tips during cryopreservation. Acta Physiol Plant 36:3189-198View Article
  
• 作者单位：Jin-Mei Zhang (1)
  Bin Huang (2)
  Xiao-Ning Zhang (3)
  Gayle M. Volk (4)
  Yuan-Chang Zhou (2)
  Xiao-Ling Chen (1)
  
  1. National Genebank, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
  2. Fujian Agriculture and Forestry University, Fuzhou, 350002, China
  3. Institute of Rice Research, JiangXi Academy of Agricultural Sciences, Nanchang, 330200, China
  4. USDA-ARS National Center for Genetic Resources Preservation, 1111 S. Mason St., Fort Collins, CO, 80521, USA
  
• 刊物主题：Plant Sciences; Cell Biology; Developmental Biology; Plant Breeding/Biotechnology; Plant Genetics & Genomics;
• 出版者：Springer US
• ISSN：1475-2689

文摘

Petunia (Petunia × hybrida Vilm.) is an important horticultural crop conserved in the National Genebank of China. Here, a droplet-vitrification cryopreservation protocol was developed for petunia shoot tips. An orthogonal array experiment and additional one-factor experiments were performed to optimize key variables, including the age of in vitro plants, the concentration of sucrose in the preculture solution, the preculture duration, the duration of osmoprotection (2.0?M glycerol and 0.4?M sucrose), the length of exposure to and concentration of plant vitrification solution 2 (30% glycerol, 15% dimethyl sulfoxide, 15% ethylene glycol, and 0.4?M sucrose), and the recovery medium. By using the combined results of the orthogonal and one-factor experiments, the droplet-vitrification procedure for petunia cultivar Niu 2 was formulated efficiently and effectively. The highest regrowth levels were obtained using the following procedure: shoot tips were dissected from in vitro plantlets that were 20?d old, precultured in MS liquid medium with 0.2?M sucrose solution for 2?d, incubated with osmoprotection solution for 30?min at 25°C, cryoprotected with PVS2 for 30?min at 0°C, and rapidly immersed in liquid nitrogen. Cryopreserved shoot tips were then diluted in MS liquid medium with 1.2?M sucrose for 20?min at 25°C and regrown on solidified MS basal medium with half concentration of NH4NO3, KH2PO4, KNO3, and sucrose. Regrowth levels were as high as 80%. No morphological changes were observed and amplification of 15 simple sequence repeats revealed no genetic alterations after cryopreservation.