Molecular cloning, characterization of CAT, and eco-toxicological effects of dietary zinc oxide on antioxidant enzymes in Eisenia fetida

详细信息    查看全文

• 作者：Wenguang Xiong (1)
  Yongxue Sun (1)
  Mengjia Zou (1)
  Rizwan-Ul-Haq Muhammad (1)
  
• 关键词：Dietary ZnO ; cDNA cloning ; Antioxidant ; Eisenia fetida ; Eco ; toxicology ; Biomarker
• 刊名：Environmental Science and Pollution Research
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：20
• 期：3
• 页码：1746-1755
• 全文大小：630KB
• 参考文献：1. Andrews GK (2001) Cellular zinc sensors: MTF-1 regulation on gene expression. Biometals 14:223-37 CrossRef
  2. Arockiaraj J, Easwvaran S, Vanaraja P, Singh A, Othman RY, Bhassu S (2012) Molecular cloning, characterization and gene expression of an antioxidant enzyme catalase ( / Mr Cat) from / Macrobrachium rosenbergii. Fish Shellfish Immun 32:670-82 CrossRef
  3. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276-87 CrossRef
  4. Bosi P, Merialdi G, Sarli G, Casini L, Gremokolini C, Preziosi R, Brunetti B, Trevisi P (2011) Effects of doses of ZnO or Zn-Glutamate on growth performance, gut characteristics, health and immunity of early-weaned pigs orally challenged with / E. coli K88. Ital J Anim Sci 2:361-63
  5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-54 CrossRef
  6. Bulbul A, Bulbul T, Kucukersan S, Sireli M, Eryavuz A (2008) Effects of dietary supplementation of organic and inorganic Zn, Cu and Mn on oxidant/antioxidant balance in laying hens. Kafkas Univ Vet Fak Derg 14:19-4
  7. Cabreiro F, Picot CR, Perichon M, Friguet B, Petropoulos I (2009) Overexpression of methionine sulfoxide reductases A and B2 protects MOLT-4 cells against zinc-induced oxidative stress. Antioxid Redox Signal 11:215-26 CrossRef
  8. Case C, Carlson M (2002) Effect of feeding organic and inorganic sources of additional zinc on growth performance and zinc balance in nursery pigs. J Anim Sci 80:1917-924
  9. Chung MJ, Walker PA, Brown RW, Hogstrand C (2005) ZINC-mediated gene expression offers protection against H₂O₂-induced cytotoxicity. Toxicol Appl Pharm 205:225-36 CrossRef
  10. Chung MJ, Hogstrand C, Lee SJ (2006) Cytotoxicity of nitric oxide is alleviated by zinc-mediated expression of antioxidant genes. Exp Biol Med 231:1555-563
  11. Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) CRC handbook of methods for oxygen radical research. CRC, Boca Raton, FL, pp 283-84
  12. Dawei A, Zhisheng W, Anguo Z (2010) Protective effects of Nano-ZnO on the primary culture mice intestinal epithelial cells in in vitro against oxidative injury. World J Agric Sci 6:149-53
  13. Feng L, Tan LN, Liu Y, Jiang J, Jiang WD, Hu K, Li SH, Zhou XQ (2011) Influence of dietary zinc on lipid peroxidation, protein oxidation and antioxidant defence of juvenile Jian carp ( / Cyprinus carpio var. Jian). Aquac Nutr 17:875-82 CrossRef
  14. Frei B (1999) Molecular and biological mechanisms of antioxidant action. FASEB J 13:963-64
  15. Gerhard GS, Kauffman EJ, Grundy MA (2000) Molecular cloning and sequence analysis of the / Danio rerio catalase gene. Comp Biochem Physiol B: Biochem Mol Biol 127:447-57 CrossRef
  16. Gioda C, Lissner L, Pretto A, Da Rocha J, Schetinger M, Neto J, Morsch V, Loro V (2007) Exposure to sublethal concentrations of Zn (II) and Cu (II) changes biochemical parameters in / Leporinus obtusidens. Chemosphere 69:170-75 CrossRef
  17. Guo H, Zhang D, Cui S, Chen M, Wu K, Li Y, Su T, Jiang S (2011) Molecular characterization and mRNA expression of catalase from pearl oyster / Pinctada fucata. Mar Genom 4:245-51 CrossRef
  18. Kashiwagi A, Kashiwagi K, Takase M, Hanada H, Nakamura M (1997) Comparison of catalase in diploid and haploid / Rana rugosa using heat and chemical inactivation techniques. Comp Biochem Physiol B: Biochem Mol Biol 118:499-03 CrossRef
  19. Kawasaki L, Aguirre J (2001) Multiple catalase genes are differentially regulated in / Aspergillus nidulans. J Bacteriol 183:1434-440 CrossRef
  20. Keller GA, Krisans S, Gould SJ, Sommer JM, Wang CC, Schliebs W, Kunau W, Brody S, Subramani S (1991) Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes. J Cell Biol 114:893-04 CrossRef
  21. Kindermann B, D?ring F, Fuchs D, Pfaffl MW, Daniel H (2005) Effects of increased cellular zinc levels on gene and protein expression in HT-29 cells. Biometals 18:243-53 CrossRef
  22. Klaine SJ, Alvarez PJJ, Batley GE, Fernandes TF, Handy RD, Lyon DY, Mahendra S, McLaughlin MJ, Lead JR (2008) Nanomaterials in the environment: behavior, fate, bioavailability, and effects. Environ Toxicol Chem 27:1825-851 CrossRef
  23. Klotz MG, Klassen GR, Loewen PC (1997) Phylogenetic relationship among prokaryotic and eukaryotic catalases. Mol Biol Evol 14(9):951-58 CrossRef
  24. LaCourse EJ, Hernandez-Viadel M, Jefferies JR, Svendsen C, Spurgeon DJ, Barrett J, John Morgan A, Kille P, Brophy PM (2009) Glutathione transferase (GST) as a candidate molecular-based biomarker for soil toxin exposure in the earthworm / Lumbricus rubellus. Environ Pollut 157:2459-469 CrossRef
  25. Liu Z, Lu L, Li S, Zhang L, Xi L, Zhang K, Luo X (2011) Effects of supplemental zinc source and level on growth performance, carcass traits, and meat quality of broilers. Poult Sci 90:1782-790 CrossRef
  26. Livingstone D, Martinez PG, Michel X, Narbonne J, O’hara S, Ribera D, Winston G (1990) Oxyradical production as a pollution-mediated mechanism of toxicity in the common mussel, / Mytilus edulis L., and other molluscs. Funct Ecol 4:415-24 CrossRef
  27. Loro VL, Jorge MB, Silva KR, Wood CM (2012) Oxidative stress parameters and antioxidant response to sublethal waterborne zinc in a euryhaline teleost / Fundulus heteroclitus: protective effects of salinity. Aquat Toxicol 110:187-93 CrossRef
  28. Luo Z, Tan X-Y, Zheng J-L, Chen Q-L, Liu C-X (2011) Quantitative dietary zinc requirement of juvenile yellow catfish / Pelteobagrus fulvidraco, and effects on hepatic intermediary metabolism and antioxidant responses. Aquaculture 319:150-55 CrossRef
  29. Ma W, Niu H, Feng J, Wang Y (2011) Effects of zinc glycine chelate on oxidative stress, contents of trace elements, and intestinal morphology in broilers. Biol Trace Elem Res 142:546-56 CrossRef
  30. Maity S, Roy S, Chaudhury S, Bhattacharya S (2008) Antioxidant responses of the earthworm / Lampito mauritii exposed to Pb and Zn contaminated soil. Environ Pollut 151:1- CrossRef
  31. Manduzio H, Monsinjon T, Galap C, Leboulenger F, Rocher B (2004) Seasonal variations in antioxidant defences in blue mussels / Mytilus edulis collected from a polluted area: major contributions in gills of an inducible isoform of Cu/Zn-superoxide dismutase and of glutathione / S-transferase. Aquat Toxicol 70:83-3 CrossRef
  32. Matés JM (1999) Antioxidant enzymes and their implications in pathophysiologic processes. Spain Front Biosci 4:339-45 CrossRef
  33. McCully G, Hill G, Link J, Weaver R, Carlson M, Rozeboom D (1995) Evaluation of zinc sources for the newly weaned pig. J Anim Sci 73:72
  34. Oteiza PI, Clegg MS, Zago MP, Keen CL (2000) Zinc deficiency induces oxidative stress and AP-1 activation in 3T3 cells. Free Radic Biol Med 28:1091-099 CrossRef
  35. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158
  36. Pan X, Zhang D, Chen X, Bao A, Li L (2011) Antimony accumulation, growth performance, antioxidant defense system and photosynthesis of / Zea mays in response to antimony pollution in soil. Water Air Soil Pollut 215:517-23 CrossRef
  37. Reinecke S, Reinecke A (2004) The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch Environ Contam Toxicol 46:208-15
  38. Ricketts H, Morgan A, Spurgeon D, Kille P (2004) Measurement of annetocin gene expression: a new reproductive biomarker in earthworm ecotoxicology. Ecotox Environ Safe 57:4-0 CrossRef
  39. Smith J, Tokach M, Goodband R, Nelssen J, Richert B (1997) Effects of the interrelationship between zinc oxide and copper sulfate on growth performance of early-weaned pigs. J Anim Sci 75:1861-866
  40. Stehbens WE (2003) Oxidative stress, toxic hepatitis, and antioxidants with particular emphasis on zinc. Exp Mol Pathol 75:265-76 CrossRef
  41. Sureda A, Box A, Tejada S, Blanco A, Caixach J, Deudero S (2011) Biochemical responses of / Mytilus galloprovincialis as biomarkers of acute environmental pollution caused by the / Don Pedro oil spill (Eivissa Island, Spain). Aquat Toxicol 101:540-49 CrossRef
  42. Van der Ploeg MJC, Baveco JM, van der Hout A, Bakker R, Rietjens IMCM, van den Brink NW (2011) Effects of C60 nanoparticle exposure on earthworms ( / Lumbricus rubellus) and implications for population dynamics. Environ Pollut 159:198-03 CrossRef
  43. Wang J, Wu C, Feng J (2011) Effect of dietary antibacterial peptide and zinc-methionine on performance and serum biochemical parameters in piglets. Czech J Anim Sci 56:30-6
  44. Wu C, Zhang W, Mai K, Xu W, Zhong X (2011) Effects of dietary zinc on gene expression of antioxidant enzymes and heat shock proteins in hepatopancreas of abalone / Haliotis discus hannai. Comp Biochem Physiol C: Pharmacol Toxicol 154:1- CrossRef
  45. Xilan Y, Gang L, Chungen W, Baoqing H, Lirong D, Pengzu P, Yanhai X (2011) A catalase from the freshwater mussel / Cristaria plicata with cloning, identification and protein characterization. Fish Shellfish Immunol 31:389-99 CrossRef
  46. Xiong W, Bai L, Muhammad R-U-H, Zou M, Sun Y (2012) Molecular cloning, characterization of copper/zinc superoxide dismutase and expression analysis of stress-responsive genes from / Eisenia fetida against dietary zinc oxide. Comp Biochem Physiol C: Pharmacol Toxicol 155:416-22 CrossRef
  47. Zeng HW, Cai YJ, Liao XR, Zhang F, Zhang DB (2011) Production, characterization, cloning and sequence analysis of a monofunctional catalase from / Serratia marcescens SYBC08. J Basic Microbiol 51:205-14 CrossRef
  
• 作者单位：Wenguang Xiong (1)
  Yongxue Sun (1)
  Mengjia Zou (1)
  Rizwan-Ul-Haq Muhammad (1)
  
  1. National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou, People’s Republic of China
  
• ISSN：1614-7499

文摘

The full-length cDNA of catalase (EfCAT) from Eisenia fetida was cloned (GenBank accession no. JN617999). Sequence characterization revealed that EfCAT protein sequence contained proximal heme-ligand signature sequence (351RLFSYSDTH359), two glycosylation sites (N145 and N436), the proximal active site signature (61FDRERIPERVVHAKGAGA78), and 12 amino acids (N145, H191, F195, S198, R200, N210, Y212, K234, I299, W300, Q302, and Y355), which were identified as putative residues involved in NADPH binding. These conserved motifs and catalase signature sequences were essential for the structure and function of EfCAT. The present study also investigated the effect of the veterinary food additive zinc oxide on antioxidant processes in E. fetida, at different concentrations and exposure durations. A significant increase (by 106.0?% compared to controls) in CAT activity at 500?mg/kg was registered at day 15. The superoxide dismutase (SOD) activity at 500?mg/kg increased to the maximum value (by 44.0?%) measured at day 15. There was a significant increase in glutathione peroxidase (GPx) activity for all concentrations after 5?days. The results showed that dietary Zn (500?mg/kg) causes oxidative damage to earthworms. At early stages of earthworms exposed to ZnO, GPx is the main enzyme to impair the oxidative status; while at later stages the enzymes CAT and SOD were the main indicators of oxidative stress. The antioxidant enzymatic variations may be an adaptive response of earthworms to survive in contaminated soils.