Effects of nitrogen source and phosphate concentration on biomass and metabolites accumulation in adventitious root culture of Glycyrrhiza uralensis Fisch

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• 作者：Shuangshuang Yin (1)
  Yao Zhang (1)
  Wenyuan Gao (1)
  Juan Wang (1)
  Shuli Man (2)
  Hui Liu (2)
  
• 关键词：Adventitious root ; Glycyrrhiza uralensis Fisch ; Nitrogen source ; Phosphate concentration ; BTBB
• 刊名：Acta Physiologiae Plantarum
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：36
• 期：4
• 页码：915-921
• 全文大小：433 KB
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• 作者单位：Shuangshuang Yin (1)
  Yao Zhang (1)
  Wenyuan Gao (1)
  Juan Wang (1)
  Shuli Man (2)
  Hui Liu (2)
  
  1. Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China
  2. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China
  
• ISSN：1861-1664

文摘

We investigated the influence of ammonia/nitrate ratio and phosphate concentration on biomass and accumulation of metabolites in adventitious roots of Glycyrrhiza uralensis Fisch. A NH4 +/NO3 ?/sup> ratio of 10:20 was optimal for the production of biomass (0.30?g dry weight) as well as polysaccharide (18.98?mg?g?) and glycyrrhetinic acid (0.31?mg?g?). The content of glycyrrhizic acid (0.47?mg?g?) and flavonoid (8.11?mg?g?) reached the optimum at an ammonia/nitrate ratio of 15:15 and 20:10, respectively. In case of phosphate concentration, a higher growth rate (9.18) and content of polysaccharide (15.66?mg?g?) was obtained at 1.25?mM phosphate concentration. However, 0.625?mM phosphate was favorable for the content of flavonoid (7.54?mg?g?) and glycyrrhizic acid (0.57?mg?g?). The content of glycyrrhetinic acid (0.32?mg?g?) reached the peak when treated with 0.3125?mM phosphate. A scale-up culture of adventitious roots was established using a balloon-type bubble bioreactor (BTBB). Maximum growth rates of 25.95- and 22.44-fold were obtained in 3 and 5 L BTBBs, respectively, which was higher than that in 0.5 L shake flask (18.36). The contents of flavonoid (7.60?mg?g?) and polysaccharide (15.09?mg?g?) reached the peak in 5 L and 3 L BTBB, respectively. The contents of glycyrrhizic and glycyrrhetinic acid were a little higher than that in BTBBs.