Structural characterization and biological activities of two α-glucans from Radix Paeoniae Alba

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• 作者：Wuxia Zhang ; Peng Li ; Dan Song ; Hong Niu ; Songshan Shi…
• 关键词：Radix Paeoniae Alba ; Polysaccharides ; Antioxidant activity ; Immunological activity
• 刊名：Glycoconjugate Journal
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：33
• 期：2
• 页码：147-157
• 全文大小：746 KB
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• 作者单位：Wuxia Zhang (1)
  Peng Li (2)
  Dan Song (1)
  Hong Niu (1)
  Songshan Shi (3)
  Shunchun Wang (3)
  Jinyou Duan (1)
  
  1. College of science, Northwest A&F University, Yangling, 712100, Shaanxi, China
  2. College of life science, Northwest A&F University, Yangling, 712100, Shaanxi, China
  3. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-4986

文摘

Radix Paeoniae Alba is widely used in Chinese traditional medicine to treat various diseases such as gastrointestinal disorders, cancer, and other diseases. In this study, two polysaccharides RPAPW1 and RPAPW2 were isolated from Radix Paeoniae Alba by DEAE-52 cellulose chromatography and G-25 sephadex. According to physicochemical methods, NMR and methylation analysis, RPAPW1 and RPAPW2 were established to be α-glucans consisting of predominant 4-linked α- Glc residues branched at O-6 and contained trace amount of protein and uronic acid. Immunological tests indicated that RPAPW1, RPAPW2 and could promote splenocyte proliferation and RAW264.7 phagocytic activity. In vitro, RPAPW1 and RPAPW2 elicited a week reducing power, DPPH scavenging activity and could not protect the PC12 cells from H₂O₂ damage. These data implied polysaccharides RPAPW1 and RPAPW2 had the potential to be a natural immunopotentiating and antioxidant supplement for preparing functional foods and nutraceuticals.