Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope

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• 作者：Shaopeng Wang ; Suhui Deng ; Xiaoqing Cai ; Shangguo Hou ; Jiajun Li…
• 关键词：telomere ; superresolution imaging ; stimulated emission depletion (STED) microscopy ; fluorescence in situ hybridization (FISH)
• 刊名：SCIENCE CHINA Chemistry
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：59
• 期：11
• 页码：1519-1524
• 全文大小：647 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Chinese Library of Science
  Chemistry
  
• 出版者：Science China Press, co-published with Springer
• ISSN：1869-1870
• 卷排序：59

文摘

The significant role of telomeres in cells has attracted much attention since they were discovered. Fluorescence imaging is an effective method to study subcellular structures like telomeres. However, the diffraction limit of traditional optical microscope hampers further investigation on them. Recent progress on superresolution fluorescence microscopy has broken this limit. In this work, we used stimulated emission depletion (STED) microscope to observe fluorescence-labeled telomeres in interphase cell nuclei. The results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal microscope. Two adjacent telomeres were clearly separated via STED imaging, which could hardly be discriminated by confocal microscopy due to the diffraction limit. We conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres.