Porcine CD4 promoters and enhancers can direct foreign gene expression in human cells

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• 作者：Xiaochen Lin (1)
  Xin Yang (1)
  Fuwang Chen (1)
  Yuhang Cao (1)
  Xiujuan Meng (1)
  Jie Jiang (1)
  Daxing Pang (1)
  Yicheng Zhao (1)
  Hongsheng Ouyang (1)
  Linzhu Ren (1)
  
• 关键词：Cluster of differentiation 4 (CD4) ; Enhancer ; Expression ; Porcine ; Promoter
• 刊名：Biotechnology Letters
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：35
• 期：5
• 页码：663-669
• 全文大小：996KB
• 参考文献：1. Adlam M, Duncan DD, Ng DK, Siu G (1997) Positive selection induces CD4 promoter and enhancer function. Int Immunol 9:877-87 CrossRef
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• 作者单位：Xiaochen Lin (1)
  Xin Yang (1)
  Fuwang Chen (1)
  Yuhang Cao (1)
  Xiujuan Meng (1)
  Jie Jiang (1)
  Daxing Pang (1)
  Yicheng Zhao (1)
  Hongsheng Ouyang (1)
  Linzhu Ren (1)
  
  1. Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, China
  
• ISSN：1573-6776

文摘

Porcine CD4 proximal promoter and enhancer sequences were cloned and aligned with the corresponding human and murine sequences. The alignment showed nucleotide homology between porcine and human sequences was 62.4?% for the CD4 promoter and 56.6?% for the CD4 enhancer. The nucleotide homology between porcine and murine sequences was 42.5?% for the CD4 promoter and 25.4?% for the CD4 enhancer. The proximal enhancer and promoter regions of the CD4 gene from porcine, murine and human cells were compared for their ability to direct foreign gene expression in transiently transfected human cell lines. The results indicated the porcine CD4 promoters and enhancers could effectively direct expression of a foreign gene in human cells. The porcine promoter was equally efficient as CMV and EF-1α in directing gene expression.