Overexpression of the RIXI xylanase inhibitor improves disease resistance to the fungal pathogen, Magnaporthe oryzae, in rice

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• 作者：Chunxiao Hou (1)
  Ting Lv (1)
  Yihua Zhan (1)
  Yaoyao Peng (1)
  Yingying Huang (2)
  Dean Jiang (1)
  Xiaoyan Weng (1)
  
• 关键词：Rice xylanase inhibitor ; Gene expression ; Plant disease resistance ; Magnaporthe oryzae
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：120
• 期：1
• 页码：167-177
• 全文大小：1,220 KB
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• 作者单位：Chunxiao Hou (1)
  Ting Lv (1)
  Yihua Zhan (1)
  Yaoyao Peng (1)
  Yingying Huang (2)
  Dean Jiang (1)
  Xiaoyan Weng (1)
  
  1. College of Life Science, Zhejiang University, Hangzhou, 310058, People鈥檚 Republic of China
  2. College of Medicine, Zhejiang University, Hangzhou, 310058, People鈥檚 Republic of China
  
• ISSN：1573-5044

文摘

In this paper we focus our attention on RIXI, a member of the XIP type xylanase inhibitor proteins in rice. RIXI-overexpression transgenic lines were generated by expressing the RIXI gene under the control of auliflower mosaic virus 35S promoter. In comparison with the wild-type (WT) plants, the transgenic plants had significantly increased levels of RIXI and showed resistance to Magnaporthe oryzae. Transgenic plants also contained higher levels of H2O2 and had larger changes in catalase and superoxide dismutase activities than the WT plants. The results showed that the increase in RIXI expression was accompanied by the up-regulation of pathogenesis-related genes and genes related to the jasmonate signaling pathway. To clarify the expression pattern of RIXI, a ProRIXI: GUS vector was constructed and transgenic rice lines were obtained. GUS staining results suggested that the RIXI gene possessed distinctive, tissue-specific and grow stage-specific expression patterns in rice. This is the first report on the expression patterns of rice xylanase inhibitors and our results provide direct evidence, at the plant level, that xylanase inhibitors are involved in plant defense against fungal pathogens.