Atoh7 promotes retinal Müller cell differentiation into retinal ganglion cells

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• 作者：Wei-tao Song ; Qi Zeng ; Xiao-bo Xia ; Kun Xia ; Qian Pan
• 关键词：Atoh7 ; Retinal ganglion cells ; Müller cells ; Progenitor cells
• 刊名：Cytotechnology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：68
• 期：2
• 页码：267-277
• 全文大小：3,687 KB
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• 作者单位：Wei-tao Song (1)
  Qi Zeng (1)
  Xiao-bo Xia (1)
  Kun Xia (2)
  Qian Pan (2)
  
  1. Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, 410008, China
  2. State Key Lab of Medical Genetics of China, Central South University, Changsha, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biomedicine
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-0778

文摘

Glaucoma is one of the leading eye diseases due to the death of retinal ganglion cells. Increasing evidence suggests that retinal Müller cells exhibit the characteristics of retinal progenitor cells and can differentiate to neurons in injured retinas under certain conditions. However, the number of ganglion cells differentiated from retinal Müller cells falls far short of therapeutic needs. This study aimed to promote the differentiation of retinal Müller cells into ganglion cells by introducing Atoh7 into the stem cells dedifferentiated from retinal Müller cells. Rat retinal Müller cells were isolated and dedifferentiated into stem cells, which were transfected with PEGFP-N1 or PEGFP-N1-Atoh7 vector, and then further induced to differentiate into ganglion cells. The proportion of ganglion cells differentiated from Atoh7-tranfected stem cells was significantly higher than that of control transfected or untransfected cells. In summary, Atoh7 promotes the differentiation of retinal Müller cells into retinal ganglion cells. This may open a new avenue for gene therapy of glaucoma by promoting optic nerve regeneration.