Colorimetric determination of cysteine by exploiting its inhibitory action on the peroxidase-like activity of Au@Pt core-shell nanohybrids

详细信息    查看全文

• 作者：Na Pan ; Wang Li-Ying ; Liang-Liang Wu ; Chi-Fang Peng ; Zheng-Jun Xie
• 关键词：Metal nanoparticles ; Enzyme mimic ; Electron paramagnetic resonance ; Lineweaver ; Burk diagram ; Michaelies ; Menten kinetics
• 刊名：Microchimica Acta
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：184
• 期：1
• 页码：65-72
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
• 出版者：Springer Vienna
• ISSN：1436-5073
• 卷排序：184

文摘

The authors report that the peroxidase-like activity of Au@Pt core-shell nanohybrids (Au@PtNHs) is selectively inhibited by cysteine. This finding has led to  a highly sensitive colorimetric assay for cysteine that is based on the nanohybrid-catalyzed oxidation of TMB by H₂O₂ to form a blue product. The method has a detection limit of 5.0 nM and a linear range from 10 nM to 20 μM. The assay is highly selective over other amino acids. It was successfully applied to the determination of cysteine in an injection containing a mixture of amino acids.