Adsorption and adhesion of blood proteins and fibroblasts on multi-wall carbon nanotubes

详细信息    查看全文

• 作者：DeJun Li (1)
  Li Yuan (1)
  Ying Yang (1)
  XiangYun Deng (1)
  XiaoYing Lü (2)
  Yan Huang (2)
  Zheng Cao (2)
  Hao Liu (3)
  XueLiang Sun (3)
  
• 关键词：multi ; wall carbon nanotubes ; cell adhesion ; protein adsorption ; toxicity ; tissue compatibility
• 刊名：Science China Life Sciences
• 出版年：2009
• 出版时间：May 2009
• 年：2009
• 卷：52
• 期：5
• 页码：479-482
• 全文大小：897KB
• 参考文献：1. Gu X T, Meng J, Kong H, et al. Protein adsorption and PEG modification on the surface of single-walled carbon nanotubes non-woven film. Chin J Biomed Eng, 2005, 24: 764-65
  2. Carrero-Sanchez J C, Elías A L, Mancilla R, et al. Biocompatibility and toxicological studies of carbon nanotubes doped with nitrogen. Nano Lett, 2006, 8: 1609-616 CrossRef
  3. Cui D, Tian F, Ozkan C S, et al. Effect of single wall carbon nanotubes on human HEK293 cells. Toxicol Lett, 2005, 155: 73-4 CrossRef
  4. Pulskamp K, Diabate S, Krug H F. Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants. Toxicol Lett, 2007, 168: 58-0 CrossRef
  5. Dumortier H, Lacotte S, Pastorin G, et al. Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells. Nano Lett, 2006, 7: 1522-528 CrossRef
  6. Zeng H, Chittur K K, Lacefield W R. Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces. Biomaterials, 1999, 20: 377-84 CrossRef
  7. Fink D J, Hutson T B, Chittur K K, et al. Quantitative surface studies of protein adsorption by infrared spectroscopy II. Quantification of adsorbed and bulk proteins. Anal Biochem, 1987, 165: 147-54 CrossRef
  8. Ying P, Yu Y, Jin G, et al. Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry. Colloids Surf B Biointerfaces, 2003, 32: 1-0 CrossRef
  
• 作者单位：DeJun Li (1)
  Li Yuan (1)
  Ying Yang (1)
  XiangYun Deng (1)
  XiaoYing Lü (2)
  Yan Huang (2)
  Zheng Cao (2)
  Hao Liu (3)
  XueLiang Sun (3)
  
  1. College of Physics and Electronic Information Science, Tianjin Normal University, Tianjin, 300074, China
  2. State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China
  3. Department of Mechanical & Materials Engineering, University of Western Ontario, London, ON, Canada
  

文摘

This article concerns the investigation of blood protein adsorption on carbon paper and multi-wall carbon nanotubes (MWCNTs). Mouse fibroblast cell adhesion and growth on MWCNTs was also studied. The results showed that fibrinogen adsorption on carbon paper was much lower than that on MWCNTs, which means that platelets readily aggregate on the surface of MWCNTs. Mouse fibroblast cells implanted on MWCNTs tended to grow more prolifically than those implanted on carbon paper. The cell concentration observed on MWCNTs increased from 1.2×105/mL for a single day culture to 2×105/mL for a 7-day culture. No toxicity reaction was observed during the culturing period. These results indicated that MWCNTs possessed excellent tissue compatibility.