Nanosized copper oxide induces apoptosis through oxidative stress in podocytes

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• 作者：Jing Xu (1)
  Zhigui Li (1)
  Pengjuan Xu (1)
  Lu Xiao (1)
  Zhuo Yang (1)
  
• 关键词：Nanosized copper oxide ; Podocytes ; Apoptosis ; ROS ; SOD ; MDA
• 刊名：Archives of Toxicology
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：87
• 期：6
• 页码：1067-1073
• 全文大小：543KB
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• 作者单位：Jing Xu (1)
  Zhigui Li (1)
  Pengjuan Xu (1)
  Lu Xiao (1)
  Zhuo Yang (1)
  
  1. College of Medicine, Nankai University, Tianjin, 300071, China
  
• ISSN：1432-0738

文摘

Nanosized copper oxide (nano-CuO) has been widely used in many fields. Recent studies have shown that nano-CuO has toxic effects on various organs, but the effects of nano-CuO on kidney remain unclear. The aim of this study was to assess whether nano-CuO can induce the apoptosis of podocytes. The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that nano-CuO decreased podocyte viability in a concentration-dependent and time-dependent manner. The apoptotic assay by flow cytometry showed that nano-CuO induced podocyte apoptosis significantly. Meanwhile, the result of flow cytometric assay gave a clear indication that nano-CuO increased reactive oxygen species (ROS) level. The measurement of superoxide dismutase (SOD) and malondialdehyde (MDA) showed that nano-CuO decreased SOD and increased MDA levels in podocytes. These results may provide basic information for the safe application of nano-CuO in the future.