摘要
建立了水产品中亚硝酸盐的流动注射检测方法。样品用饱和硼砂提取、亚铁氰化钾和乙酸锌脱脂净化后,用滤纸过滤取上清液,用流动注射法进行检测,外标法定量,检测结果与紫外可见分光光度法保持一致。亚硝酸盐在0.0~0.250mg/L的质量浓度范围内与吸光度呈良好的线性关系,相关系数R为0.999 9,方法检出限(3S/N)为0.81 mg/kg。实际样品不同加标浓度的加标回收率在81.0%~105.8%之间,相对标准偏差为0.48%~6.56%(n=6),批内相对标准偏差均小于7%。本方法简单、快速,具有较好的准确度和精密度,适合大批量的水产品检测要求。
A method for determination of nitrite in aquatic products using continuous flow injection analysis has been developed. The samples were extracted saturated sodium borate, and purified with potassium ferri-cyanide and zinc acetate, then the supernatants obtained through filter paper were analysed by flow injection analysis instrument, using external standard method for quantitative. The results were consistent with UV visi-ble spectrophotometry. Linear relationships between values of absorbance and mass concentration of nitrite was in range of 0.0-0.250 mg/L, with correlation coefficient more than 0.999 9. The limit of detection(3 S/N) for nitrite was 0.81 mg/kg The relative recoveries of spiked nitrite at different concentration levels in real samples were 81.0%-105.8%, and with relative standard deviations of 0.48%-6.56%(n=6). The relative standard deviation in the batch is less than 7%. The method is proved to be rapid, accurate, efficient, and especially for large quantities of aquatic products samples analysis.
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