摘要
目的研究被转化生长因子β活化长链非编码RNA(LncRNA-ATB)对人上皮性卵巢癌细胞增殖、迁移和侵袭的影响。方法通过荧光实时定量聚合酶链反应(qRT-PCR)检测人正常卵巢组织细胞系及上皮性卵巢癌系A2780和OVCAR3中LncRNA-ATB表达。将OVCAR3细胞系分为对照组、LncRNA-ATB NC组和转染LncRNA-ATB inhibitor组,采用MTT试剂盒检测细胞增殖,Transwell法检测细胞迁移及侵袭能力,Western blot检测下调LncRNAATB表达对TGF-β表达的影响,qRT-PCR法检测TGF-β处理对LncRNA-ATB表达的影响。结果与正常卵巢细胞组相比,上皮性卵巢癌A2780组、OVCAR3组LncRNA-ATB相对表达量显著升高(P<0.05);与A2780组相比,OVCAR3组LncRNA-ATB相对表达量升高(P<0.05);转染后与空白组、LncRNA-ATB NC组相比,LncRNA-ATB inhibitor组LncRNA-ATB表达、细胞增殖、迁移和侵袭能力显著降低(P<0.05);下调LncRNA-ATB表达对TGF-β的表达无影响(P>0.05);TGF-β处理后LncRNA-ATB表达显著降低(P<0.05)。结论 LncRNA-ATB在上皮性卵巢癌细胞中高表达,下调LncRNA-ATB后抑制上皮性卵巢癌细胞增殖、迁移和侵袭能力。
Objective To study the effects of transforming growth factorβ-activated long non-coding RNA(LncRNA-ATB)on proliferation,migration,and invasion of human epithelial ovarian cancer cells.MethodsThe expression of LncRNA-ATB in human normal ovarian tissue cell lines and epithelial ovarian cancer lines A2780 and OVCAR3 was detected by realtime fluorescence quantitative polymerase chain reaction(qRT-PCR).The OVCAR3 cell lines were divided into control group,LncRNA-ATB NC group,and LncRNA-ATB inhibitor transfection group.Cell proliferation was detected by MTT kit.Transwell assay was used to detect cell migration and invasion.Western blot was used to detect LncRNA-ATB and TGF-βexpression.qRT-PCR method was used to detect the effect of TGF-βtreatment on the expression of LncRNA-ATB.Results Compared with the normal ovarian cell group,the relative expression of LncRNA-ATB in A2780 group and OVCAR3 group increased significantly(P<0.05);compared with A2780 group,the relative expression of LncRNA-ATB increased in OVCAR3 group(P<0.05).Compared with blank group and LncRNA-ATB NC group,LncRNA-ATB expression,cell proliferation,migration,and invasion abilities in LncRNA-ATB inhibitor group were significantly decreased(P<0.05).Down-regulation of LncRNA-ATB expression had no effect on the expression of TGF-β(P>0.05);the expression of LncRNA-ATB decreased significantly after treatment with TGF-β(P<0.05).ConclusionLncRNA-ATB was highly expressed in epithelial ovarian cancer cells.Down-regulation of LncRNA-ATB could inhibit proliferation,migration,and invasion of epithelial ovarian cancer cells.
引文
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