摘要
为进行水稻苗期耐镉胁迫QTL的定位研究,以籼稻品种昌恢121为轮回亲本、粳稻品种越光为供体亲本构建的染色体片段代换系(CSSL)群体,共97个家系为试验材料,利用质量浓度为17 mg/L的CdCl2溶液,采用水培方法进行水稻幼苗镉胁迫处理,以不加CdCl2的水培液作为对照,以苗长、根长、苗鲜重、根鲜重、苗干重、根干重等6个性状的耐镉胁迫指数为耐镉指标,检测到7个LOD>2.5的耐镉胁迫相关QTL,分别分布在水稻的第1、4、7、8、10号染色体上,贡献率在9.84%～24.50%。其中包括2个根长耐镉胁迫QTL,3个根鲜重耐镉胁迫QTL,根干重、苗干重QTL各1个。贡献率最大的是位于7号染色体上的根鲜重耐镉胁迫QTL,贡献率为24.50%,LOD值为4.82。不同形态性状间未检测到相同的QTL区域。
A CSSL population derived from a backcross between indica variety Changhui 121(as recurrent parent)and japonica variety Koshihikari(as donor parent),was used to identify QTL for Cd tolerance at the seedling stage of rice.Two parents and 97 lines were phenotyped for Cd tolerance by growing them in nutrient so?lution with a concentration of 17 mg/L CdCl2.The nutrient solution without CdCl2 was used as a control.Six traits,including resistance index of seedling length,root length,seedling fresh weight,root fresh weight,seed?ling dry weight,and root dry weight,were used as phenotypic traits.Seven QTL related to cadmium tolerance with LOD>2.5 were detected on chromosome 1,4,7,8 and 10 of rice,respectively,which explained 9.84%～24.50% of the phenotypic variance.These QTL included 2 QTL for tolerance index of root length,3 QTL for tol?erance index of seedling fresh weight,and 1 QTL for tolerance index of root dry weight and seedling dry weight,respectively.The QTL on chromosome 7 for tolerance index of seedling fresh weight had the largest contribution rate and explained up to 24.50% of the phenotypic variances,with LOD value of 4.82.No same QTL region was detected among the different phenotypic traits.
引文
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