摘要
[目的]筛选出可与解淀粉芽孢杆菌WH1G菌株复配的最佳化学药剂及比例,明确复配药剂对草莓灰霉病菌的增效作用和田间防效。[方法]采用菌丝生长速率法测定8种杀菌剂对草莓灰霉病菌的毒力,平板菌落计数法测定杀菌剂与WH1G菌株的生物相容性,Horsfall法评价复配组合的增效作用,确定复配方案并进行田间验证。[结果]氟啶胺对草莓灰霉病菌的毒力最强,与WH1G菌株有较好的生物相容性。0.0454 mg/L氟啶胺与8.8126×106cfu/m L的WH1G体积比为4∶6时,毒性比率为1.412,表现为增效作用,田间防效高达69.79%,显著高于单剂防效。[结论]氟啶胺可与解淀粉芽孢杆菌WH1G复配协同防治草莓灰霉病,2者按体积比4:6复配不仅可以提高防效,还能有效减少化学药剂的使用量。
[Aims] The study aims to screen out the optimal fungicides and ratio mixed with Bacillus amyloliquefaciens WH1 G, meanwhile to confirm the synergistic effect and control efficacy of the mixture in the fields on strawberry gray mould. [Methods] The toxicities of 8 fungicides against strawberry gray mould were tested via the measurement of the colony diameter, plate culture method was used to determine the biocompatibility between each fungicide and WH1 G.The synergistic effects of mixture and the compound scheme were determined by Horsfall method, and confirmed through field trials. [Results] The toxicity of fluazinam is the strongest among the 8 fungicides, and fluazinam showed a favorable biocompatibility with WH1 G. By combining 0.0454 mg/L fluazinam with 8.8126 ×106 cfu/m L WH1 G fermentation broth under the volume ratio of 4 ∶6, the toxicity ratio was 1.412, the control efficacy was 69.79% in the field against strawberry gray mould, which exhibited additive effects. [Conclusions] Fluazinam can be combined with WH1 G to control strawberry gray mould, the compound not only can reduce the usage of chemicals, but also promote the biocontrol efficacy under the volume ratio of 4∶6.
引文
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