摘要
为研究中华鲟免疫分子CD22的结构及其可能对应的功能,克隆中华鲟CD22 cDNA(CsCD22),进行生物信息学分析,根据结构推测功能。结果表明,成功克隆中华鲟CD22 cDNA序列,该序列全长1 748 bp,包含1个1 170 bp的开放阅读框,共编码389个氨基酸,包含3个Ig超家族结构域和3个潜在的N-糖基化位点。综上所述,中华鲟CD22分子具有高度保守的结构特征,推测其具有与其他动物CD22分子相似的免疫学功能。
In order to study the structure of CD22 and its possible corresponding functions,the Acipenser sinensis CD22 cDNA(CsCD22)was cloned and analyzed by bioinformatics.The results showed that the CD22 cDNA sequence of Acipenser sinensis was successfully cloned.The full length of the sequence was 1 748 bp and it contained a 1 170 bp open reading frame encoding a total of 389 amino acids,including three Ig superfamily domains and three potential N-glycosylation sites.In summary,the Acipenser sinensis CD22 molecule has a highly conserved structural feature and is presumed to have immunological functions similar to those of other animal CD22 molecules.
引文
[1]邓子新,陈峰.微生物学[M].北京:高等教育出版社,2017:245.
[2]徐洪来,肖敏,刘强,等.CD22分子机制的研究进展[J].医学综述,2015,21(15):2720-2722.
[3] ERE譙O-ORBEA,J,SICARD,T,CUI,H,et al.Molecular basis of human CD22 function and therapeutic targeting[J]. Nature Communications,2017,8(1):1-11.
[4] DEPOIL,D,DUSTIN,M L.Agile CD22 nanoclusters run rings around fenced BCR[J].The EMBO Journal,2016,35(3):237-238.
[5] CHEN,J,WANG,H,XU,W,et al.Besides an ITIM/SHP-1-dependent pathway,CD22 collaborates with Grb2 and plasma membrane calciumATPase in an ITIM/SHP-1-independent pathway of attenuation of Ca2+i signal in B cells[J].Oncotarget,2016,7(35):56129-56146.
[6]葛新顺,廖晓龙.CD22分子的生物学功能研究进展[J].交通医学,2007(1):24-26.
[7]吴剑峰.中华鲟的过去,现在和将来[J].生物学教学,1993(7):36-38.
[8]云文.“活化石”:中华鲟[J].中国钓鱼,1995(2):41.
[9]张辉,危起伟.命运堪忧的中华鲟[J].大自然,2016(6):8-11.
[10]常剑波,曹文宣.中华鲟物种保护的历史与前景[J].水生生物学报,1999(6):712-720.
[11] HUANG Z,WANG L.Yangtze Dams increasingly threaten the survival of the Chinese sturgeon[J].Curr Biol,2018,28(22):3640-3647.
[12]肖慧.葛洲坝水利枢纽与珍稀鱼类保护的实践[M]//黄真理.21世纪长江大型水利工程中的生态与环境保护.北京:中国环境科学出版社,1998:199-209.
[13]刘榴,石君玲,尤修玲,等.大鲵泛素结合酶cgsUBE2L3基因克隆及其在免疫应答中的表达分析[J].安徽农业科学,2017,45(22):93-97.
