摘要
为研究MS-222对鳊鱼保活运输的影响,分析比较了在不同质量浓度MS-222(0、20、40、60、80、100 mg/L和120 mg/L)、不同水温(2、7、15、20℃)和不同鱼水质量比(2∶1、1∶1、1∶3、1∶5)下鳊鱼的存活率,从而确定及筛选最佳的保活运输条件,并检测和分析在保活运输试验中水质指标和鱼体生化指标的变化和影响。试验结果显示,当MS-222质量浓度为60 mg/L、水温7℃、鱼水比1∶3时,保活时间最长,鱼体存活率最高;随着保活时间的延长,水体指标中氨氮含量、微生物、pH均呈上升趋势,而溶解氧水平则呈下降趋势;在鱼体指标中,肌肉中糖原、pH下降,乳酸含量上升;在血液指标中乳酸脱氢酶、谷草转氨酶、血糖、尿素和肌酐均显著增加(P<0.05),说明随着保活时间的延长,鱼体肝脏和肾脏组织代谢水平受到一定的影响,但其变化幅度均小于对照组。研究结果表明,在鳊鱼保活过程中可适当使用MS-222麻醉剂以延长存活时间和提高存活率。
The effect of MS-222 on the survival of bream Parabramis pekinensis was studied during transport in this paper. Survival rates of bream in different concentrations of MS-222(0, 20, 40, 60, 80, 100 and 120 mg/L),different water temperatures(2, 7, 15, 20 ℃) and different fish-water mass ratios(2∶1,1∶1,1∶3 and 1∶5)were studied.Therefore, the keep-alive transportation conditions were optimized, and the changes and effects of water quality indicators and fish biochemical indicators in the keep-alive transport test were detected and analyzed.The results showed that the longest survival time and the highest survival rate were obtained under the concentration of 60 mg/L of anesthetic solution, the 7 ℃ of water temperature and the ratio of fish to water=1∶3. With the extension of the keep-alive time, the content of ammonia nitrogen, and microorganism and pH in the water were shown to be an upward trend, while the dissolved oxygen levels decreased. The glycogen contents and pH in the muscle were decreased, and the content of lactic acid was increased in the fish index. Lactic dehydrogenase, aspertate aminotransferase, and blood glucose activities, and urea and creatinine levels were significantly increased in the blood index(P<0.05), indicating that the metabolic level of hepatopancreas and kidney tissues was affected with the prolongation of survival time, but the change were smaller than those of the control group. The findings showed that the anesthetic MS-222 was used properly to prolong the survival time and improve the survival rate of the bream.
引文
[1] 农业部渔业渔政管理局.2017中国渔业统计年鉴[M].北京:中国农业出版社,2017.
[2] Iwama G K,Pickering A D,Sumpter J P,et al.Fish stress and health in aquaculture[M].London:Cambridge University Press,2011:35-72.
[3] King H R.Fish transport in the aquaculture sector:an overview of the road transport of Atlantic salmon in Tasmania[J].Journal of Veterinary Behavior:Clinical Applications and Research,2009,4(4):163-168.
[4] 刘骁,谢晶,黄硕琳.鱼类保活运输的研究进展[J].食品与发酵工业,2015,41(8):255.
[5] 张晓林,王秋荣,刘贤德.鱼类保活运输技术研究现状及展望[J].渔业现代化,2017,44(1):40-44.
[6] Topic Popovic N,Strunjak-Perovic I,Coz-Rakovac R,et al.Tricaine methane-sulfonate (MS-222) application in fish anaesthesia[J].Journal of Applied Ichthyology,2012,28(4):553-564.
[7] 何蓉,谢晶.水产品保活技术研究现状和进展[J].食品与机械,2012(5):243-246.
[8] Pramod P K,Ramachandran A,Sajeevan T P,et al.Comparative efficacy of MS-222 and benzocaine as anaesthetics under simulated transport conditions of a tropical ornamental fish Puntius filamentosus (Valenciennes)[J].Aquaculture Research,2010,41(2):309-314.
[9] Schnick R R.Zero withdrawal anesthetic for all finfish and shellfish:need and candidates[J].Fisheries,2006,31(3):122-126.
[10] Gilderhus P,Marking L.Comparative efficacy of 16 anesthetic chemicals in rainbow trout [J].North American Journal of Fisheries Management,1987,7(2):288-292.
[11] 魏友海.鳊鱼的营养价值与食用[J].科学养鱼,2009(11):78-78.
[12] Marking L L,Meyer F P.Are better anesthetics needed in fisheries[J].Fisheries,1985,10(6):2-5.
[13] 刘长琳,何力,陈四清,等.鱼类麻醉研究综述[J].渔业现代化,2007,34(5):21-25.
[14] 杨家林,蒋艳,蔡德建.大獭蛤低温保活技术的初步研究[J].广西科学院学报,2006,22(2):75-77.
[15] 郭丰红,汪之和.MS-222对鳜鱼成鱼麻醉效果的研究[J].湖南农业科学,2009(7):150-153.
[16] 李春梅,黄永坚.MS-222麻醉金鱼的研究[J].黄冈职业技术学院学报,2002,4(4):67-70.
[17] 王秀华,张烨伟,杨春志.MS-222对牙鲆麻醉效果[J].渔业科学进展,2009,30(3):1-3.
[18] 王利娟,程守坤,张饮江,等.MS-222在加州鲈鱼模拟运输中的麻醉效果[J].上海海洋大学学报,2015,24(2):235-241.
[19] Vinagre C,Madeira D,Narciso L,et al.Effect of temperature on oxidative stress in fish:lipid peroxidation and catalase activity in the muscle of juvenile seabass,Dicentrarchus labrax[J].Ecological Indicators,2012,23(4):274-279.
[20] 龙华.温度对鱼类生存的影响[J].中山大学学报:自然科学版,2005,44(B06):254-257.
[21] 丁永良.活鱼储运机械化[J].渔业机械仪器,1993(6):23-27.
[22] 张瑞霞,张娟,熊善柏,等.低温处理对鲫生化特性及肉质的影响[J].华中农业大学学报,2008,27(4):532-535.
[23] 刘永波,王雅倩.氨氮对养殖鱼类的危害及其控制方法[J].渔业致富指南,2016(4):59-60.
[24] 叶英植,吴伟力,陈仅星.渔业养殖水中溶解氧的测控系统研制[J].仪器仪表学报,2004,25(增刊):178-179.
[25] 国家环境保护局.GB 11607—89,中华人民共和国国家标准渔业水质标准[S].北京:中国标准出版社,1989.
[26] 邢峰杰,王晓波,郑振魁,等.对水质检测中菌落计数结果质量控制的探讨[J].中国水利,2015(1):38-40.
[27] 李黎,曹振东,付世建.力竭性运动后鲇鱼幼鱼乳酸、糖原和葡萄糖水平的变动[J].水生生物学报,2007,31(6):880-885.
[28] Pavlidis M,Angellotti L,Papandroulakis N,et al.Evaluation of transportation procedures on water quality and fry performance in red porgy (Pagrus pagrus) fry[J].Aquaculture,2003,218(1):187-202.
[29] 董淑丽,王占彬,雷雪芹,等.热应激对动物血液生化指标的影响[J].家畜生态,2004,25(2):54-56.
[30] 侯俊利,庄平,冯琳,等.铅暴露与排放对中华鲟幼鱼血液中ALT、AST活力的影响[J].生态环境学报,2009,18(5):1669-1673.
[31] 冯广朋,庄平,章龙珍,等.温度对中华鲟幼鱼代谢酶和抗氧化酶活性的影响[J].水生生物学报,2012,36(1):137-142.
[32] 周玉,郭文场,杨振国,等.鱼类血液学指标研究的进展[J].上海水产大学学报,2001,10(2):163-165.
[33] 尾崎久雄著.鱼类血液与循环生理[M].许学龙,熊国强,缪圣赐译.上海:上海科学技术出版社,1982.
[34] 林浩然.鱼类生理学[M].广州:广东高等教育出版社,1999.
