摘要
发根农杆菌Ri质粒作为一种天然存在的植物遗传转化系统,已在植株再生,品种改良,增强植物抗逆性以及生产次生代谢产物等方面得到广泛应用,并取得了巨大的经济和社会效益.全文概述了发根农杆菌Ri质粒介导的植物基因工程原理、方法、影响因素、应用前景及存在的问题.
The root-inducing plasmid of Agrobacterium rhizogenes,as a natural plant genetic transformation system,is widely applied in plant regeneration,variety improvement,stress resistance and secondary metabolites production,and achieves great economic and social benefits.The principles,methods,affecting factors,prospect and problems of the plant genetic technologies with Ri plasmid of Agrobacterium rhizogenes are reviewed.
引文
[1]SMITH E F,TOWNSEND C O.A plant-tumor of bacterial origin[J].Science,1907,25(643):671-673.
[2]KREGTEN M V,PATER S de,ROMEIJN R,et al.T-DNA integration in plants results from polymerase-θ-mediated DNA repair[J].Nature Plants,2016,2(11):16164.
[3]MAISTRENKO O M,LUCHAKIVSKA Y S,ZHOLOBAK N M,et al.Obtaining of the transgenic Heliantus tuberosus L.plants,callus and“hairy”root cultures able to express the recombinant human interferon alpha-2bgene[J].Cytology and Genetics,2015,49(5):308-313.
[4]JOUANIN L.Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids[J].Plasmid,1984,12(2):91-102.
[5]PETIT A,DAVID C,DAHL G A,et al.Further extension of the opine concept:Plasmids in Agrobacterium rhizogenes cooperate for opine degradation[J].Molecular Genetics and Genomics.,1983,190(2):204-214.
[6]VILAINE F,CASSE-DELBART F.Independent induction of transformed roots by the TL and TR regions of the Ri plasmid of agropine type Agrobacterium rhizogenes[J].Molecular Genetics and Genomics,1987,206(1):17-23.
[7]SATUTI N N,MORIGUCHI K,SATO M,et al.Genome structure of Ri plasmid(3).Sequencing analysis of the vir region of pRi1724in Japanese Agrobacterium rhizogenes[J].Nucleic Acids Symposium,2000,44(1):95-96.
[8]MORIGUCHI K,MAEDA Y,SATOU M,et al.The complete nucleotide sequence of a plant root-inducing(Ri)plasmid indicates its chimeric structure and evolutionary relationship between tumor-inducing(Ti)and symbiotic(Sym)plasmids in Rhizobiaceae[J].Journal of Molecular Biology,2001,307(3):771-784.
[9]LIN J,ZHOU B,YANG Y,et al.Piercing and vacuum infiltration of the mature embryo:a simplified method for Agrobacteriummediated transformation of indica rice[J].Plant Cell Reports,2009,28(7):1065-1074.
[10]HOSOKAWA K,MATSUKI R,OIKAWA Y,et al.Genetic transformation of gentian using wild-type Agrobacterium rhizogenes[J].Plant Cell,Tissue and Organ Culture,1997,51(2):137-140.
[11]VILAINE F,CASSE-DELBART F.A new vector derived from Agrobacterium rhizogenes plasmids:a micro-Ri plasmid and its use to construct a mini-Ri plasmid[J].Gene,1987,55(1):105-114.
[12]SRIPRIYA R,SANGEETHA M,PARAMESWARI C,et al.Improved Agrobacterum-mediated co-transformation and selectable marker elimination in transgenic rice by using a copy number pBIN19-derived binary vector[J].Plant Science,2011,180(6):766-774.
[13]RADUTOIU S,MADSEN L H,MADSEN E B,et al.Agrobacterium rhizogenes pRi TL-DNA integration system:agene vector for Lotus japonicus transformation[M].Berlin:Springer Netherlands,2005:285-287.
[14]ZHOU H,CHEN S,LI X,et al.Generating marker-free transgenic tobacco plants by Agrobacterium-mediated transformation with double T-DNA binary vector[J].Acta Botanica Sinica,2003,45(9):1103-1108.
[15]SUKHAPINDA K,SPIVEY R,SIMPSON R B,et al.Transgenic tomato(Lycopersicon esculentum L.)transformed with a binary vector in Agrobacterium rhizogenes:Non-chimeric origin of callus clone and low copy numbers of integrated vector T-DNA[J].Molecular Genetics and Genomics,1987,206(3):491-497.
[16]GANGOPADHYAY M,DEWANJEES,BHATTACHARYYA S,et al.Effect of different strains of Agrobacterium rhizogenes and nature of explants on Plumbago indica hairy root culture with special emphasis on root biomass[J].Natural Product Communications,2010,5(5):1913-1916.
[17]胡霄.不同发根农杆菌菌株诱导番茄毛状根的研究[J].广东农业科学,2015,42(3):20-23.
[18]周济铭.农杆菌敏感小麦基因型筛选与转化条件的优化[J].种子,2016,35(5):31-35.
[19]孙洁,黄团,李加纳,等.农杆菌培养方式和预培养基激素配比对甘蓝型油菜下胚轴转化效应分析[J].西南大学学报(自然科学版),2007,29(12):49-53.
[20]DOVE H,MAYES R W.Plant wax components:a new approach to estimating intake and diet composition in herbivores[J].Journal of Nutrition,1996,126(1):13-26.
[21]NEDELA V,TIHLARIKOVA E,SCHIEBERTOVA P.Study of plant waxes using low temperature method for ESEM[J].Microscopy&Microanalysis,2016,22(3):1180-1181.
[22]THILIP C,RAJU S C,VARUTHARAJU K,et al.Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera(L.)Dunal using sonication and heat treatment[J].Biotech,2015,5(6):949-956.
[23]GUREL S,GURELl E,KAUR R,et al.Efficient,reproducible Agrobacterium-mediated transformation of sorghum using heat treatment of immature embryos[J].Plant Cell Reports,2009,28(3):429-444.
[24]YU K W,MURTHY H N,HAHN E J,et al.Ginsenoside production of hairy root cultures of Panax ginseng:influence of temperature and light[J].Biochemical Engineering Journal,2005,23(1):53-56.
[25]GODWIN I,TODD G,FORD-LLOYD B,et al.The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species[J].Plant Cell Reports,1991,9(12):671-675.
[26]KODAHL N,MULLER R,LUTKEN H.The Agrobacterium rhizogenes oncogenes rolB and ORF13increase formation of generative shoots and induce dwarfism in Arabidopsis thaliana(L.)Heynh[J].Plant Science,2016,252(2):22-29.
[27]SHI H,WANG B,YANG S,et al.Induction of hairy roots of dianthus chinensis and its plant regeneration[J].Chinese Bulletin of Botany,2016,23(3):92-97.
[28]敖小平.侣基因转化大红甜橙的研究[D].长沙:湖南农业大学,2009.
[29]施和平.五寸石竹毛状根诱导及其植株再生[J].植物学报,2016,51(3):363-368.
[30]徐洪伟.发根农杆菌诱导玉米毛状根再生植株及抗旱性研究[D].长春:东北师范大学,2007.
[31]张鑫,郝玉琴.RNA干扰技术的研究进展[J].中国麻风皮肤病杂志,2016,32(2):119-122.
[32]刘鹏,王帅帅,杨高山,等.农杆菌介导瞬时沉默小麦HCP1的研究[J].中国农业科学,2015,48(20):4188-4196.
[33]许娜,崔晓霞,郭娜,等.发根农杆菌介导的大豆DAD基因沉默嵌合植株的获得[J].大豆科学,2015,34(4):576-581.
[34]AL-SHALABI Z,DORAN P M.Biosynthesis of fluorescent CdS nanocrystals with semiconductor properties:Comparison of microbial and plant production systems[J].Journal of Biotechnology,2016,223(10):13-23.
[35]GAI Q Y,JIAO J,LUO M,et al.Establishment of hairy root cultures by Agrobacterium rhizogenes mediated transformation of Isatis tinctoria L.For the efficient production of flavonoids and evaluation of antioxidant activities[J].PloS One,2015,10(3):e0119022.http://doi.org/10.1371/journal.pone.0119022.
[36]赵寿经,杨振堂,李昌禹,等.发根农杆菌诱导人参产生发根及离体发根中人参皂甙含量的测定[J].吉林农业大学学报,2001,23(2):57-63.
[37]赵生琴.西兰花毛状根的诱导及扩繁体系的建立[D].兰州:甘肃农业大学,2015.
[38]付晓.甜叶菊毛状根培养体系的建立及绿原酸类化合物的积累研究[D].南昌:江西农业大学,2015.
