摘要
利用同源重组技术构建了溶藻弧菌(Vibrio alginolyticus) ZJ-T小RNA srvg17985的缺失突变株,并比较研究了野生株和srvg17985突变株在LBS中的生长特性、运动性、胞外蛋白酶分泌、对铁的吸收利用、对抗生素的抗性以及生长代谢等生物学特性。结果表明,小RNA srvg17985缺失后不影响溶藻弧菌在LBS中的生长、运动性、胞外蛋白酶分泌、对铁的吸收利用、对抗生素的抗性以及对测定的多数碳源、氮源的代谢;但srvg17985突变株对昆布多糖(laminarin)、果胶(pectin)以及二羟基丙酮(dihydroxyacetone)的利用增强,并转变为可利用丙氨酸-天冬氨酸(Ala-Asp)作为单一氮源。
We constructed the sRNA srvg17985 knock-out mutant in Vibrio alginolyticus ZJ-T by using homologous recombination, so as to compare the biological characteristics of growth in LBS medium, motility, extracellular protease secretion, iron utilization, antibiotic resistance and metabolism between the wild type and the mutant strains. The results show that the absence of sRNA srvg17985 did not affect the growth in LBS medium, motility, extracellular protease secretion, iron utilization, antibiotic resistance and most of the tested carbon and nitrogen sources metabolism in V. alginolyticus. However, the mutant showed better utilization of laminarin, pectin and dihydroxyaceton, and gained the ability to utilize alanine-aspartic acid(Ala-Asp) as the sole nitrogen source.
引文
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