摘要
目的探究白藜芦醇对小鼠皮层神经元兴奋性的影响。方法取1月龄雄性小鼠制备小鼠急性大脑前额叶皮层切片后,随机分为空白对照组与白藜芦醇组。实验过程中对照组不做处理,白藜芦醇组灌流液加入终浓度为100μmol·L~(-1)的白藜芦醇溶液。采用电生理全细胞记录模式分别记录两组神经元的兴奋性突触后电流(sEPSC)、动作电位(AP)以及Cl~-电流。结果与对照组比较,白藜芦醇组兴奋性电流幅度(Amplititude)与频率(Frequency)均降低,梯度电流刺激诱发的AP数目减少,Cl~-电流的幅度与频率也明显降低。结论白藜芦醇具有抑制小鼠皮层神经元兴奋性的作用,其机制可能是抑制神经元兴奋性电流的作用优于抑制Cl~-电流的作用。
Objective To study the effects of resveratrol on the excitability of mouse cortical neurons. Methods The acute prefrontal cortex slices of mice were prepared from 1-month old male mice,and randomly divided into control group and resveratrol group. With electrophysiological recording, the resveratrol group was treated with a final concentration of 100 μmol·L~(-1) of resveratrol solution while the control group was not treated. The spontaneous excitatory postsynaptic current(sEPSC),action potential(AP)and the chloride current of the two groups of neurons were recorded respectively. Results Compared to the control group, the amplitude and frequency of excitatory current in the resveratrol group were reduced,and the number of AP induced by gradient current stimulation was also decreased, the amplitude and frequency of chloride current were also significantly reduced. Conclusion Resveratrol can inhibit the excitability of mouse cortical neurons. The mechanism may be the inhibition to the excitatory current of neurons is stronger than that of chloride current.
引文
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