摘要
制备了一种基于液晶取向变化的非标记液晶型免疫传感器,用于检测天蚕素B。首先采用硅烷化试剂自组装膜法构建基底敏感膜诱导液晶分子呈均一垂直排列,再通过戊二醛交联法将天蚕素B固定到基底表面,当天蚕素B抗体与天蚕素B特异性结合后扰乱了液晶分子的取向,使液晶膜的颜色和亮度发生变化,以此实现对天蚕素B的检测,检测限可达0.1 ng/m L。本方法具有灵敏度高、特异性好、非标记和操作简单等优点。
A label-free liquid crystal immunosensor based on orientation changes of liquid crystals for the detection of cecropin B was reported.The mixed self-assembled monolayers formed by both long and short alkyls could cause uniform vertical alignment of liquid crystals.The homeotropic-to-tilted transition of liquid crystals induced by the specific binding event of anti-cecropin B antibody and cecropin B immobilized via a cross-linker glutaradehyde could result in the corresponding changes of the color and brightness of liquid crystal film and as a result,the limit of cecropin B detection is as low as 0.1 ng/m L.This study offers a simple,highly sensitive and specific,lable-free method for cecropin B detection.
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