摘要
为了解鸳鸯茉莉(Brunfelsiaacuminata)花色变化的机理,采用高效液相色谱(HPLC)体系检测其开花过程中花青素组成的变化。结果表明,优化的HPLC体系为:流速为0.8 mL min–1,流动相A为7.5%甲酸乙腈,流动相B为7.5%甲酸水,洗脱程序为0 min,8%A;15 min,18%A;25 min,23%A;45 min,40%A;50 min,8%A。利用优化体系检测到鸳鸯茉莉花瓣中含有锦葵色素-3-O-葡萄糖苷、矮牵牛素葡萄糖苷和飞燕草素葡萄糖苷3种花青苷,其中锦葵色素-3-O-葡萄糖苷的含量最高,飞燕草素葡萄糖苷含量最低,且在花色由深变浅的过程中3种花青苷的含量均降低。因此,鸳鸯茉莉的呈色与这3种花青苷有关,且锦葵色素-3-O-葡萄糖苷起主导作用。
In order to understand the mechanism of flower colour change of Brunfelsia acuminate, the changes in composition of anthocyanins in petals were studied by using high performance liquid chromatography(HPLC) system. The results showed that the optimized HPLC system was following: the flow rate was 0.8 mL min–1, 7.5% formic acid acetonitrile as mobile phase A and 7.5% formic acid as mobile phase B; the elution program was 0 min with 8% A; 15 min with 18% A; 25 min 23% A; 45 min with 40% A and 50 min with 8% A. There were three kinds of anthocyanins, such as malvidin-3-O-glucoside chloride, petunidin-3-glucoside and delphinidin-3-glucoside in petals of B. acuminata by using the optimized system. Among them, the content of malvidin-3-Oglucoside chloride was the highest, followed by petunidin-3-glucoside and the content of delphinidin 3-glucoside was the lowest. Moreover, the contents of three kinds of anthocyanin decreased with the petal color from dark to light. Therefore, it was indicated that the coloration of B. acuminata is related to these three anthocyanins, and the malvidin-3-O-glucoside chloride plays a dominant role.
引文
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