摘要
目的:探索益肾健脾止血法对阿托伐他汀诱导的斑马鱼脑出血的影响。方法:用阿托伐他诱导Fli1∶EgFP/gAtA1∶dsRed双转基因斑马鱼脑出血,建立脑出血模型,分别给予不同浓度的三子补血汤,在荧光显微镜下拍照观察,计算斑马鱼脑出血面积和出血率。结果:与模型组相比,三子补血汤250、125μg/m L浓度组脑出血发生率有差异(P﹤0.05);与模型组相比,三子补血汤250、125、62.5、31.25μg/mL浓度组脑出血面积有差异(P﹤0.05)。结论:益肾健脾止血法可降低斑马鱼脑出血发生率,改善斑马鱼脑出血面积。
Objective: To investigate the effect of Yishen Jianpi Zhixue therapy on zebrafish's cerebral hemorrhage induced by atorvastatin.Methods: The cerebral hemorrhage of Fli1: EGFP/GATA1: dsRed doubletransgenic zebrafish was induced by atorvastatin.The models of cerebral hemorrhage were established and given the different concentrations of Sanzi Buxue decoction respectively.Cerebral hemorrhage area and hemorrhage rate of zebrafish were observed and calculated by photograph under fluorescence microscope.Results: Compared with model group,the cerebral hemorrhagerates in groups of 250 and 125μg/mL concentrations of Sanzi Buxue decoction were different(P<0.05).Compared with model group,the cerebral hemorrhage areas in groups of 250,125,62.5 and31.25μg/m L concentrations of Sanzi Buxue decoction were different(P<0.05).Conclusion: Yishen Jianpi Zhixue therapy can reduce the incidence ofcerebral hemorrhage of zebrafish,and improve the cerebral hemorrhage area of zebrafish.
引文
[1]温春苗.脑出血患者并发症的预防与护理体会[J].中国实用神经疾病杂志, 2016(1):134-135.
[2]中华医学会神经病学分会,中华医学会神经病学分会脑血管病学组中国脑出血诊治指南(2014)[J],中华神经科杂志, 2015,48(6):435-444.
[3]蔺彩娟,甘欣锦.自拟三子补血汤治疗化疗后骨髓抑制的经验[J].西部中医药, 2012, 25(10):47-49.
[4]蔺彩娟,甘欣锦.三子补血汤对化疗后血小板减少小鼠tNF-α和tgF-β1血清水平的影响[J].山西中医, 2014, 30(11):44-47.
[5]傅华,甘欣锦,杨文忠,等.益肾健脾止血法干预急性白血病化疗后血小板减少临床研究[J].山西中医, 2015, 31(9):17-19.
[6]Vera Binder, Leonard I. Zon. highthroughput in vivo p henotyping:the zebrafis h as tool for drugdiscovery for hematopoietic stemcells and cancer. DrugDiscovery today:Disease Models, 2013, Vol.10(1), pp.e17-e22
[7]Jagadeeswaran P,S hee han JP,C haigFE,et al.Identificationand c haracterization of zebrafis hthrombocytes.Br J haematol, 1999, 107(4), 731-738
[8]LangMR,gihrg,gawazRajpuMP,etal.hemostasisin Daniorerio:isthe zebrafis h a useful model for platelet researc h.Jthromb haemost, 2010, 8(6), 1 159-1 169
[9]周忠焱,徐佳琪,赵外荣,等.斑马鱼脑出血模型的建立及丹参在该模型上改善脑出血的研究.中华中医药学刊, 2017,35(7):1121-1125.
[10]于莹莹,尹起亮,吴荻.中药防治恶性肿瘤化疗后骨髓抑制研究概况[J].中国医药导报, 2017, 14(26):41-44.
[11]张娜,曹勇.恶性肿瘤化疗毒副反应从脾肾论治[J].新中医, 2010, 42(10):1-2.
[12]张惟郁,沈小珩.益气养血升血小板方治疗化疗后血小板减少症的临床观察40例.中国医药指南, 2012,10(4):223-226.
[13]王艳艳,翁志国,马艳辉,等.益气补肾健脾方治疗小儿慢性难治性血小板减少性紫癜临床研穷.西部中医药, 2017,30(7):1-3.
[14]夏小军,段赞.中医药治疗免唼牲血小板减少性紫德的思路与方法[J].西部中医药, 2016,29(3):41-44.
[15]左晶晶.化疗后血小板减少的治疗进展[J].湖北中医杂志, 2011, 33(12):77-78.
[16]陆清昀,戴小军,常娜,等.健脾养血浓煎剂防治消化道肿瘤患者化疗后骨髓抑制疗效观察[J].中医药临床, 2018,30(2):352-355.
[17]秦丽,许成勇,王毓国,等.应用益气养血、健脾补肾法治疗化疗后骨髓抑制[J].中医杂志, 2017, 58(12):1 017-1020.
[18]尹凯明,甘欣锦.化疗后血小板减少的中医治疗进展[J].甘肃中医, 2009,22(8):71-73.
[19]梅全喜.现代中药药理手册[M].北京:中国中医药出版社,1998:585-596.
[20]孙燕,谷铣之.肿瘤综合治疗的原则和实践[J].中国肿瘤,1999,8(1):21-23.
[21]孙静.仙鹤草不同成分促进小鼠化疗后血小板减少作用研究[J].中医药信息, 2013,30(2):17-19.
[22]甘欣锦,贾新颜,杨文忠,等.补肾健脾止血法对化疗后血小板减少的影响[J].中国中医急症, 2010,19(4):595-597.
