摘要
目的分析河北地区女性人乳头瘤病毒(HPV)感染率及感染亚型情况。方法回顾性分析2014年4月到2017年8月来河北省人民医院妇科门诊就诊的26 385例患者,采用荧光定量PCR法对患者宫颈脱落细胞样本进行21种HPV亚型的检测,不同年龄组HPV感染率比较采用卡方检验。结果 26 385例受检者中,HPV感染率为33.05%(8 719/26 385),其中高危型HPV感染率为26.50%(6 992/26 385),阳性患者中占比80.19%(6992/8719);共计检出21种HPV亚型,12 901例次HPV感染,其中高危型HPV感染11 059例次,占比91.46%,感染前三位依次为HPV16、HPV58、HPV52,均为高危亚型;15~25岁和≥56岁年龄组HPV感染率较高,分别与其他三组比较差异均有统计学意义(χ~2=203.281,P<0.01;χ~2=42.033,P<0.01)。结论妇科门诊患者HPV感染率较高,以高危型HPV感染为主,主要感染型别为HPV16、HPV58、HPV52。15~25岁和≥56岁年龄组HPV感染率相对较高。
Objective To analyze the infection rate and subtypes of female human papillomavirus(HPV)in Hebei.Methods A retrospective analysis was conducted on 26 385 patients in the gynecological outpatient clinic of Hebei People′s Hospital from April 2014 to August 2017.The method of qPCR was used to detect the genotypes of HPV in the specimens of exfoliated cervical cells of the patients.The rates of HPV infections in different age groups were analyzed by using chi-square test.Results Among the 26 385 patients,the HPV infection rate was 33.05%(8 719/26 385),among which the high-risk HPV infection rate was26.50%(6 992/26 385),accounting for 80.19% of the positive patients(6 992/8 719).A total of 21 types of HPV subtypes were detected,involving 12 901 cases of HPV infection which included 11 059 cases of high-risk HPV infection,accounting for 91.46% of the total cases.The top three subtypes were HPV16,HPV58 and HPV52,all of which were high-risk subtypes.The HPV infection rates were higher in age groups of 15-25 years and ≥56 years,with statistically significant differences from other age groups(χ~2=203.281,P<0.01;χ~2=42.033,P<0.01).Conclusion The HPV infection rate was high in gynecological outpatients,with high-risk HPV16,HPV58 and HPV52 being predominant.A higher rate of HPV infection can be seen in patients at ages of 15-25 and≥56 years.
引文
[1]de SanjoséS,Brotons M,Pavón MA.The natural history of human papillomavirus infection[J].Best Pract Res Clin Obstet Gynaecol,2017,47:2-13.
[2]Hancock G,Hellner K,Dorrell L.Therapeutic HPV vaccines[J].Best Pract Res Clin Obstet Gynaecol,2018,47:59-72.
[3]Berti FCB,Pereira APL,Cebinelli GCM,et al.The role of interleukin 10in human papilloma virus infection and progression to cervical carcinoma[J].Cytokine Growth Factor Rev,2017,34:1-13.
[4]Serrano B,Brotons M,Bosch FX,et al.Epidemiology and burden of HPV-related disease[J].Best Pract Res Clin Obstet Gynaecol,2018,47:14-26.
[5]Adams TS,Mbatani NH.Clinical management of women presenting with field effect of HPV and intraepithelial disease[J].Best Pract Res Clin Obstet Gynaecol,2018,47:86-94.
[6]Basu P,Mittal S,Bhadra Vale D,et al.Secondary prevention of cervical cancer[J].Best Pract Res Clin Obstet Gynaecol,2018,47:73-85.
[7]ZHI Yanfang,XU Xuefeng,LI Xiaofu,et al.Genotype distribution of human papillomavirus infection in cervical intraepithelial neoplasia and cervical squamous cells cancer in Henan province[J].J Harbin Med Univ,2014,48(2):142-146.(in Chinese)智艳芳,徐雪峰,李肖甫,等.河南地区宫颈上皮内瘤变及宫颈癌患者HPV感染型别分析[J].哈尔滨医科大学学报,2014,48(2):142-146.
[8]CHENG Hao.Latest developments in HPV detection and immunotherapy[J].China Med Abstract Dermatol,2017,34(1):84-95.(in Chinese)程浩.HPV检测和免疫治疗进展[J].中国医学文摘·皮肤科学,2017,34(1):84-95.
[9]Patty NJS,van Dijk HM,Wallenburg I,et al.To vaccinate or not to vaccinate?Perspectives on HPV vaccination among girls,boys,and parents in the Netherlands:a Q-methodological study[J].BMC Public Health,2017,17(1):872.
[10]王卡娜,郄明蓉.HPV治疗性疫苗研究现状[J].实用妇产科杂志,2017,33(2):89-90.
[11]JIAO Jie,YU Yang,WU Lan,et al.An analysis of HPV genotyping in cervical cells of 9515women in Nanjing district[J].Jiangsu Med J,2017,43(1):14-16.(in Chinese)焦杰,俞杨,邬兰,等.南京地区9515例女性宫颈细胞HPV基因分型分析[J].江苏医药,2017,43(1):14-16.
[12]CAO Shiru,HUANG Qian,HUO Xiangran,et al.Human papilloma virus infection situation in 13933gynecological outpatients[J].Chin J Microecol,2017,29(9):1080-1083.(in Chinese)曹诗茹,黄茜,霍向然,等.13933例妇科门诊患者人乳头瘤病毒感染状况分析[J].中国微生态学杂志,2017,29(9):1080-1083.
[13]CHEN Shufen,WEI Shuli,SONG Chunlin,et al.Genotyping of 8 985cases of HPV infection in Foshan[J].J Trop Med,2015,15(3):325-327.(in Chinese)陈淑芬,峗淑莉,宋春林,等.佛山地区8 985例HPV感染亚型分析[J].热带医学杂志,2015,15(3):325-327.
