摘要
以棕榈油皂粒为皂基制备熊果酸香皂,按照国家标准方法对香皂中游离苛性碱、水分和挥发物含量进行检测,采用平板计数法和动物实验对该香皂的体外抑菌作用和安全性进行研究。结果表明,熊果酸香皂对白色假丝酵母、肺炎克雷伯氏菌、伤寒沙门氏菌、铜绿假单胞菌、MRSA1和MRSA2均具有明显抑菌作用,在熊果酸质量分数为7.5%时抑菌效果最为显著,动物实验表明香皂对小鼠皮肤和眼睛黏膜无不良反应和毒副作用,长期使用不会对皮肤产生刺激性。
Ursolic acid soap was prepared by adding ursolic acid into palm oil base. The contents of free caustic alkali, water and volatiles were determined by national standard method. Plate counting and animal experiments were used to study the in vitro antibacterial effect and the safety of the manual soap. The results showed that the ursolic acid soap had obvious bacteriostatic action against Canidia Albicans, Pseudomonas aer uginosa, Salmonella Enterica, Klebsiella Pneumoniae, MRSA1, and MRSA2. The antibacterial effect was the most remarkable with 7.5% mass fraction of ursolic acid. Animal experiments showed that the ursolic acid soap had no adverse effects on the skin and eye mucosa of mice, indicative of no irritation in the skin under long-term use.
引文
[1]王鹏,张忠义,吴忠.熊果酸在药用植物中的分布及药理作用[J].中药材,2000,23(11):717-722.
[2]BARICEVIC D,SOSA S,DELLA L R,et al.Topical antiinflammatory activity of Salvia officinalis L.leaves:the relevance of ursolic acid[J].Journal of Ethnopharmacology,2001,75:125-132.
[3]NELSON A T,CAMELIO A M,CLAUSSEN K R,et al.Synthesis of oxygenated oleanolic and ursolic acid derivatives with anti-inflammatory properties[J].Bioorganic&Medicinal Chemistry Letters,2015,25(19):4342-4346.
[4]JESUSJ A,LAGO JH,LAUREN TIMD,et al.Antimicrobial activity of oleanolic and ursolic acids:an update[J].Evidence-Based Complementray and Alternative Medicine,2015,20:620.
[5]WANG C M,JHAN Y L,TSAI S J,et al.The pleiotropic antibacterial mechanisms of ursolic acid[J].Molecules,2016,21(7):884.
[6]姜敏.秦巴山区秦艽中熊果酸的提取及性质研究[J].时珍国医国药,2016(4):839-843.
[7]孟艳秋,陈瑜,王趱.熊果酸的研究进展[J].中国新药杂志,2007(1):29-32.
[8]张晓蓉,陈功锡,徐定华,等.新型黄花蒿保健香皂工艺研制及品质评价[J].天然产物研究与开发,2013,52(11):1550-1554.
[9]中华人民共和国国家发展和改革委员会.QB-T 2623.1-2003肥皂试验方法肥皂中游离苛性碱的测定[S].北京:中国标准出版社,2008.
[10]中华人民共和国国家发展和改革委员会.QB-T 2623.4-2003.肥皂试验方法肥皂中水分和挥发物含量的测定烘箱法[S].北京:中国标准出版社,2008.
[11]李华.平板菌落计数的改进方法[J].生物学通报,2006,41(1):51.
[12]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 21606-2008.化学品急性经皮毒性试验方法[S].北京:中国标准出版社,2008.
[13]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 21609-2008.化学品急性眼刺激性/腐蚀性试验方法[S].北京:中国标准出版社,2008.
[14]中华人民共和国国家发展和改革委员会.QB/T 2485-2008.香皂[S].北京:中国标准出版社,2008.
