摘要
采用硅胶柱色谱、Sephadex LH-20柱色谱等色谱方法,对台东荚蒾乙酸乙酯部位进行分离,从中分离纯化得到7个三萜类化合物,根据其波谱数据和理化性质鉴定为3β,6β-二羟基齐墩果-11,13(18)-二烯-28-酸(1)、3β-羟基齐墩果-11,13(18)-二烯-28-酸(2)、12-烯-齐墩果-28-酸-3β-棕榈酸酯(3)、3β-乙酰古柯二醇(4)、科索酸(5)、熊果醇(6)和熊果酸(7)。其中,化合物1为新化合物,其绝对构型通过单晶衍射得以证实。化合物2～5为首次从荚蒾属中分离得到。采用LPS诱导的RAW264.7释放NO的细胞模型,评价了化合物1～7的体外抗炎活性,结果显示,与阳性对照槲皮素(IC_(50)值为25.46±0.62μmol·L~(-1))相比,化合物5显示出较强的NO抑制活性,其IC_(50)值为25.52±0.56μmol·L~(-1)。
The chemical constituents of Viburnum taitoense Hayata were investigated using column chroma‐tography silica gel and Sephadex LH-20, etc. Seven pentacyclic triterpenoids were isolated and their structures were elucidated by spectral data and physicochemical properties as 3β,6β-dihydroxy olean-11,13(18)-dien-28-acid(1), 3β-hydroxy olean-11,13(18)-diene-28-acid(2), 12-ene-olean-28-acid-3β-palmitate(3), 3β-acetylcocodiol(4),corosolic acid(5), uvaol(6) and ursolic acid(7). Among them, compound 1 is a new oleanane type triterpenoid and its absolute configuration was confirmed by single-crystal X-ray diffraction data. Compounds 2-5 were isolated from this genus for the first time. All the compounds were evaluated for their anti-inflammatory activities in vitro,and compound 5 showed significant inhibitory activity against nitric oxide(NO) production in lipopolysaccharide(LPS)-induced RAW264.7 macrophage cells with an IC_(50) of 25.52 ± 0.56 μmol·L~(-1) when compared to the positive control, quercetin(IC_(50) of 25.46 ± 0.62 μmol·L~(-1)).
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