摘要
基于甲砜霉素对典型的鲁米诺-H_2O_2化学发光系统有着明显的增敏作用,并协同流动注射分析的技术,创建了测定甲砜霉素的一种新方法。在实验的优化条件下,甲砜霉素在1.0×10~(-10)～1.0×10~(-8) mol·L~(-1)区间内的变化值与化学发光强度的相对值具备很好的线性关系,此方法对甲砜霉素的测定展现出很高的灵敏度,检出限(LOD)低至8.1×10~(-11) mol·L~(-1)(3σ)。对1.0×10~(-9) mol·L~(-1)的甲砜霉素连续11次进行了测定,其相对标准偏差(RSD)为0.34%,采用此方法对牛奶样品进行了加标回收试验,回收率在98.6%～101.5%。所提出的方法可用于牛奶中甲砜霉素的检测,这证明了其在食品样品的快速和经济成本有效检测中的应用潜力。
Based on the strong effect of thiamphenicol on luminol-H_2O_2 chemiluminescence system,a novel flow injection-chemiluminescence(FI-CL)method for the determination of thiamphenicol(TAP)is established by flow injection analysis.Under the optimal experimental conditions,there is a good linear relationship between the relative intensity of chemiluminescence and the concentration of TAP in the range of 1.0×10~(-10)～1.0×10~(-8) mol·L~(-1),with the limit of detection(LOD) as low as 8.1×10~(-11) mol·L~(-1).The value of relative standard deviation(RSD) is 0.34% for the determination of 1.0×10~(-9) mol·L~(-1) TAP(n=11).The milk sample is determined by the proposed method and the recoveries range from 98.6% to 101.5%.Thus,the application for TAP detection in milk proves its potential for rapid and cost-effective detection of food samples.
引文
[1] WANG M X,ZHANG Y X,GUO P Y.Effect of florfenicol and thiamphenicol exposure on the photosynthesis and antioxidant system of Microcystis flos-aquae[J].Aquatic Toxicology,2017(186):67-76.
[2] ZHU T,LI G Z,ROW K H.Deep eutectic solvents for the purification of chloromycetin and thiamphenicol from milk[J].Journal of Separation Science,2017,40(3):625-634.
[3] Commission Regulation(EU) N°37/2010 of 22 dec 2009:on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin[S].Offcial Journal of the European Communication,2010,L15:1-72.
[4] SHEN J,XIA X,JIANG H Y,et al.Determination of chloramphenicol,thiamphenicol,florfenicol,and florfenicol amine in poultry and porcine muscle and liver by gas chromatography-negative chemical ionization mass spectrometry[J].Journal of Chromatography B,2009(877):1523-1529 .
[5] HUANG S F,GAN N,LIU H,et al.Simultaneous and specific enrichment of several amphenicol antibiotics residues in food based on novel aptamer functionalized magnetic adsorbents using HPLC-DAD[J].Journal of Chromatography B,2017(1060):247-254.
[6] THOMPSON C S,TRAYNOR I M,FODEY T L,et al.Screening method for the detection of residues of amphenicol antibiotics in bovine,ovine and porcine kidney by optical biosensor[J].Talanta,2017(172):120-125.
[7] ZHE W,HUI Y,WEI S,et al.UPLC-MS/MS determination of thiamphenicol in human plasma and its application to a pharmacokinetic study[J].Journal of Chromatography B,2014(967):235-239.
[8] TAO X Q,HE Z F,CAO X Y,et al.Approaches for the determination of florfenicol and thiamphenicol in pork using a chemiluminescent ELISA[J].Analytical Methods,2015,7(19):8386-8392.
[9] JOSE A,MURILLO P,LUISA F,et al.Determination of Antioxidant Activity of Hibiscus Flowers by Flow Injection Analysis with Chemiluminescence Detection[J].Analytical Letters,2017,50(1):186-196.
[10] YANNA L,LI J B,WANG Y H,et al.A chemiluminescence biosensor for the detection of thrombin based on the aptamer composites[J].Spectrochimca Actapart A:Molecular and Biomolecular Spectroscopy,2018(192):153-158.
[11] SHENG Y Y,YANG H L,WANG Y,et al.Silver nanoclusters-catalyzed luminol chemiluminescence for hydrogen peroxide and uric acid detection[J].Talanta,2017(166):268-274.
[12] 郭晶,李晓璐,易钢,等.金纳米催化鲁米诺-铁氰化钾化学发光体系测定克拉维酸钾[J].分析试验室,2017,36(1):100-104.
[13] HANAN Y N,ELDAR B A,BAUER C R.Remarkable Enhancement of Chemiluminescent Signal by Dioxetane-Fluorophore Conjugates:Turn-ON Chemiluminescence Probes with Color Modulation for Sensing and Imaging[J].Journal of the American Chemical Society,2016,138(40):13438-13446.
[14] 张玉,杨春艳,舒砚勤,等.流动注射-化学发光法测定河水中对苯二酚[J].分析试验室,2016,35(3):283-286.
[15] ASGHAR M,YAQOOB M,NA B A.Chemiluminescent determination of cyromazine in milk samples using copper(III) chelate-Triton X-100 by flow injection analysis[J].Chemical Research in Chinese Universities,2017,33(3):354-359.
[16] VAKH C,EVDOKIMOVA E,POCHIVALOV A,et al.Anovel flow injection chemiluminescence method for automated and miniaurized determination of phenols in smoked food samples[J].Food Chemistry,2017(237):929-935.
[17] LI Q Q,ZHANG L J,LI J G,et al.Nanomaterial-amplified chemiluminescence systems and their applications in bioassays[J].Trac-trend in Analytical Chemistry,2011,30(2):401-413.
[18] WU N L,LUO Z M,GE Y H,et al.A novel surface molecularly imprinted polymer as the solid-phase extraction adsorbent for the selective determination of ampicillin sodium in milk and blood samples[J].Journal of Pharmaceutical Analysis,2016,6 (3):157-164.
