摘要
以甜瓜高代自交系M10-03为材料,在2叶1心期对甜瓜苗模拟淹水处理,在外源激素IAA、GA、ABA、NPA、PP_(333)、STD诱导下对甜瓜不定根生理特性的影响进行分析。结果表明,随激素处理浓度升高,幼苗不定根数经IAA处理后先降后升,在浓度为1.0 ng/L时达到峰值;GA处理甜瓜幼苗不定根的生根数和发生区域均大于CK,且呈下降趋势,NPA对不定根发生区域的影响不大;PP_(333)处理甜瓜幼苗不定根生根数及发生区域先升高后降低,在浓度为100 mg/L时达到峰值;ABA处理后甜瓜幼苗不定根数先降后升,在25 ng/L时为低值;STD处理呈先升后降趋势,在25 ng/L处达到峰值,不定根发生区域长度为对照的1.85倍。激素及激素抑制剂均能增强甜瓜幼苗根系活力,IAA和STD处理甜瓜幼苗根系活力均升高;施加GA和PP_(333)时,甜瓜幼苗根系活力先升后降,在浓度100 mg/L时达到峰值。甜瓜幼苗真叶叶绿素a、叶绿素b的含量均呈下降趋势,PP_(333)(100 mg/L)及STD(25 ng/L)处理甜瓜幼苗真叶叶绿素a和叶绿素b含量最低;ABA处理对叶绿素影响不明显。甜瓜幼苗真叶类胡萝卜素含量经IAA处理后升高,NPA、PP_(333)、ABA、STD处理后先高后低,GA处理后呈降低趋势。
引文
[1]雷加富.中国林业资源报告[M].北京:中国林业出版社,1996.
[2]谈广鸣,胡铁松.变化环境下的涝渍灾害研究进展[J].武汉大学学报(工学版),2009,42(5):565-571.
[3]王彩洁.黄淮海地区主栽大豆品种耐涝性比较[J].农家顾问,2016(8):24.
[4]禹华芳,李益得,刘高永,等.苗期水涝胁迫对不同花生品种生长性状及产量的影响[J].分子植物育种,2016(1):233-238.
[5]郑佳秋,郭军,梅燚,等.辣椒幼苗形态及生理特性对涝害胁迫的响应[J].西南农业学报,2016,29(3):536-540.
[6]Chen H J,Qualls R G,Miller G C.Adaptive responses of Lepidium latifolium to soil flooding:biomass allocation,adventitious rooting,aerenchyma formation and ethylene production[J].Environmental and Experimental Botany,2002,48(2):119-128.
[7]Shimamura S,Yoshida S,Mochizuki T.Cortical aerenchym formation in hypocotyl and adventitious roots of Luffa cylindrical subjected to soil flooding[J].Annals of Botany,2007,100(7):1431-1439.
[8]王文泉,郑永战,梅鸿献,等.不同耐渍基因型芝麻在厌氧胁迫下根系的生理与结构变化[J].植物遗传资源学报,2003,4(3):214-219.
[9]Li S W,Xue L,Xu S J,et al.Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings[J].Environmental and Experimental Botany,2009,65(1):63-71.
[10]Steffens B,Wang J,Sauter M.Interactions between ethylene,gibberellin and abscisic acid regulate emergence and growth rate of adventitious Roots in deep-water rice[J].Planta,2006,223(3):604-612.
[11]Jackson M B,Saker L R,Crisp C M,et al.Ionic and pH signaling from roots to shoots of flooded tomato plants in relation to stomatal closure[J].Plant and Soil,2003,253(1):103-113.
[12]Visser E J W,Voesenek L A C J.Acclimation to soil flooding-sensing and signal-transduction[J].Plant and Soil,2005,254(1/2):197-214.
[13]Sairam R K,Kumutha D,Ezhilmathi K,et al.Physiology and biochemistry of waterlogging tolerance in plants[J].Biologia Plantarum,2008,52(3):401-412.
[14]张志勇,卜晶晶,王素芳,等.冠菌素对不同钾水平下TTC法测定的棉花根系活力的影响[J].植物生理学报,2015,51(5):695-701.
[15]明华,胡春胜,张玉铭,等.浸提法测定玉米叶绿素含量的改进[J].玉米科学,2007(4):93-95,99.
[16]张立军,樊金娟.植物生理学实验教程[M].北京:中国农业大学出版社,2007:94-96.
[17]Pagnussat G C,Lanteri M L,Lamattina L.Nitric oxide and cyclic GMP are messengers in the indole acetic acid-induced adventitious rooting process[J].Plant Physiology,2003,132:1241-1248.
[18]Liu H,Wang S,Yu X,et al.ARL1,a LOB-domain protein required for adventitious root formation in rice[J].The Plant Journal,2005,43(1):47-56.
[19]Kitomiy Y,Ogawa A,Kitanoh I,et al.CRL4 regulates crown root formation through auxin transport in rice[J].Plant Root,2008,2:19-28.
[20]Liu S P,Wang J R,Wang L,et al.Adventitious root formation in rice requires OsGNOM1 and is mediated by the OsPINs family[J].Cell Research,2009,19(9):1110-1119.
[21]Zhao Y,Hu Y F,Dai M Q,et al.The WUSCHEL-Related homeobox gene WOX11 is required to activate Shoot-Borne crown root development in rice[J].The Plant Cell,2009,21(3):736-748.
[22]Gutierrez L,Bussell J D,Pacurar D I,et al.Phenotypic plasticity of adventitious rooting in Arabidopsis is controlled by complex regulation of AUXIN RESPONSE FACTOR transcripts and MicroRNA abundance[J].Plant Cell,2009,21(10):3119-3132.
[23]张丹凤.淹水胁迫下microRNA介导的玉米不定根生长及抗氧化机制研究[D].保定:河北农业大学,2009.