摘要
荧光光谱分析技术具有灵敏度高、检测快速等优点,三维荧光光谱提供的指纹荧光信息比普通荧光光谱更丰富,选择性更好,在多组分分离上更具优势.离散三维活体荧光光谱法通过提取水体蓝藻、绿藻、硅藻、甲藻和隐藻5个门类藻类荧光光谱的指纹特征,分类测量藻类叶绿素a浓度,经过加和得到总的水体叶绿素a浓度.将基于该方法研制的三维荧光光谱水体藻类原位测量仪用于太湖水体叶绿素a浓度的测定,并与YSI多参数水质检测仪、BBE藻类现场分析仪、分光光度法等测定结果对比,结果表明:该方法与分光光度法间无显著性差异,与分光光度法、BBE法间的相关性好,相关系数达0.96以上,精密度、准确率优于基于普通荧光法的原位测量仪,是一种快速有效的原位测量方法.
Fluorescence spectroscopy had the advantages of high sensitivity and fast detecting. The three-dimensional( 3 D) fluorescence spectrometry was more informative,better selectivity,more advantage than the general fluorescence spectroscopy on the multicomponent separation. The determination method of chlorophyll-a in water based on the discrete three-dimensional fluorescence spectrometry in vivo was studied. It extracted different fluorescence fingerprint properties of five divisions of algae( Cyanophyta,Chlorophyta,Bacillariophyta,Dinophyta and Cryptophyta),measured chlorophyll-a concentration of every algae and got the total chlorophyll-a concentration by plus. The three-dimensional fluorescence algae in-situ analyzer( AFA) was manufactured based on it. The chlorophyll-a concentration in Lake Taihu was measured by AFA. The measured values were compared with YSI,BBE and the spectrophotometry. It showed no significant difference between AFA and the spectrophotometry. There were good correlation among AFA,spectrophotometry and BBE. The correlation coefficient was above of 0.96. AFA was better precision and accuracy than the general fluorescence spectroscopy. It was a fast and effective in-situ method.
引文
[1]"Water and wastewater monitoring and analysis method"editorial board of State Environmental Protection Administration of China ed.Monitoring and analysis methods of water and wastewater:fourth edition.Beijing:China Environmental Science Press,2002:670-671.[国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法:第4版.北京:中国环境科学出版社,2002:670-671.]
[2]Almela L,Fernandez-Lopez JA,Roca MJ.High-performance liquid chromatographic screening of chlorophyll derivatives produced during fruit storage.Journal of Chromatography A,2000,870:483-489.
[3]Hu J,Liu X,Wang L et al.RP-HPLC determination of photosynthetic pigments.Marine Sciences,2011,35(11):19-28.[胡俊,柳欣,王磊等.应用反相高效液相色谱定性和定量浮游植物光合色素.海洋科学,2011,35(11):19-28.]
[4]Xu JG,Wang ZB eds.Fluorescence analysis method:3th Edition.Beijing:Science Press,2006:15-16.[许金钩,王尊本.荧光分析法:第3版.北京:科学出版社,2006:15-16.]
[5]Xiang QQ,Tang HQ,Zhu LH et al.Rapid determination of chlorophyll-a in lake water by direct fluorophotometry.Journal of Wuhan University of Technology,2011,33(11):112-115.[相青青,唐和清,朱丽华等.直接荧光分光光度法快速测定水体中叶绿素a.武汉理工大学学报,2011,33(11):112-115.]
[6]Chen LF,Zheng F.Application of rapid determination of algal biomass by chlorophyll fluorescence technique.City and Town Water Supply,2007,(6):51-52.[陈丽芬,郑锋.叶绿素荧光技术快速测定水体藻类生物量的应用.城镇供水,2007,(6):51-52.]
[7]Chen L,Sun JX.Rapid and simultaneous determination of chlorophyll a and chlorophyll b by fluorophotometry.Sichuang Environment,2013,32(3):20-23.[陈立,孙静娴.荧光光度法同时快速测定水体中叶绿素a和叶绿素b.四川环境,2013,32(3):20-23.]
[8]Cui J,Cui JS.Preparation of chlorophyll fluoresecence detector and its performance research.Hebei Chemical Engineering and Industry,2012,35(6):72-75.[崔静,崔建升.海水叶绿素荧光测定仪的研制及性能研究.河北化工,2012,35(6):72-75.]
[9]Ding JQ,Zhang JY.Application of YSI6600 for Cyanobacteria bloom early warning in Taihu Lake.The Administration and Technique of Environmental Monitoring,2011,23(1):67-70.[丁建清,张军毅.YSI6600传感器在太湖蓝藻预警工作中的应用.环境监测管理与技术,2011,23(1):67-70.]
[10]Liu Y,Chen YW,Deng JM.Discussion on accuracy and errors for phytoplankton chlorophyll-a concentration analysis using YSI(Multi-parameter water analyzer).J Lake Sci,2010,22(6):965-968.DOI:10.18307/2010.0622.[刘苑,陈宇炜,邓建明.YSI(多参数水质检测仪)测定叶绿素a浓度的准确性及误差探讨.湖泊科学,2010,22(6):965-968.]
[11]Song T,Zhu BC,Yan F.Environmental monitoring and forewarning,2016,8(5):14-18.[宋挺,朱冰川,严飞.YSI6600V2水质多参数仪现场快速法与实验室分光光度法测定湖泊藻类叶绿素的比较.环境监控与预警,2016,8(5):14-18.]
[12]Li YP,Huang LJ,Yan X.Comparative research on monitoring the content of chlorophyll-a in the water body of Dianchi Lake by hydrolab DS5 multi-parameter instrument and acetone pectrophotometry.Environmental Science Survey,2015,34(5):88-91.[李娅萍,黄丽娟,颜翔.Hydrolab DS5水质多功能监测仪与丙酮分光光度法测定滇池水体中叶绿素a.环境科学导刊,2015,34(5):88-91.]
[13]Chen Y,Zhao YY,Pan SY.Classifying diffident kinds of algae and determining their chlorophyll by PHYTO-PAM.Modern Scientific Instruments,2009,4:100-103.[陈元,赵洋甬,潘双叶.PHYTO-PAM对浮游植物中叶绿素的分类测定.现代科学仪器,2009,4:100-103.]
[14]Wang YF,Yang SB,Liu DY.Comparison of different methods for phytoplankton chlorophyll-a concentration measurement:A study case of water in Baiyangdian area.Journal of China Hydrology,2014,34(5):57-60.[王玉芳,杨士斌,刘得银.浮游植物叶绿素a含量不同方法测定比较——以白洋淀区域水体为例.水文,2014,34(5):57-60.]
[15]Zhou J,Hu ZJ,Li PP.Comparison between spectrophotometry and bbe-fluoroprobe for measuring chlorophyll a—illustrated by the example of Qiandao Lake.Journal of Hydroecology,2013,34(2):80-84.DOI:10.15928/j.1674-3075.2013.02.011.[周静,胡忠军,李培培.基于叶绿素a测定的分光光度法与bbe法比较:以千岛湖为例.水生态学杂志,2013,34(2):80-84.]
[16]Jin XC,Tu QY eds.Specification for investigation of lake eutrophication:2th Edition.Beijing:China Environmental Science Press,1990:268-270.[金相灿,屠清英.湖泊富营养化调查规范:第2版.北京:中国环境科学出版社,1990:268-270.]
[17]Wu MY.Comparisons on detection methods of chlorophyll a.Journal of Fujian Fisheries,2011,33(4):61-63.[吴妹英.叶绿素a测定方法之比较.福建水产,2011,33(4):61-63.]
[18]Han GC,Gu F,Zhang ZC.Discussing about chlorophyll a determination method in fresh water.Environmental Monitoring in China,2005,21(1):55-57.[韩桂春,谷丰,张忠臣.淡水中叶绿素a测定方法的探讨.中国环境监测,2005,21(1):55-57.]
[19]Zhang G,Hu H,Xie F.Discussing about“chlorophyll a determination method”in the determination of productivity.Environmental Study and Monitoring,2006,19(1):20-21.[张帼,虎华,谢翡.对生产力测定中“叶绿素a测定”方法的探讨.环境研究与监测,2006,19(1):20-21.]
[20]Yin GF,Zhao NJ,Hu L et al.Classified measurement of phytoplankton based on characteristic fluorescence of photosynthetic pigments.Acta Optica Sinica,2014,34(9):312-317.DOI:10.3788/AOS201434.0930005.[殷高方,赵南京,胡丽等.基于色素特征荧光光谱的浮游植物分类测量方法.光学学报,2014,34(9):312-317.]
[21]Lv PY,Cui JS,Duan LL.Effect of turbidity on fluorescence intensity of chlorophyll-a in water.Physical Testing and Chemical Analysis Part B:Chemical Analysis,2015,51(6):741-744.DOI:10.11973/lhjy-hx201506002.[吕鹏翼,崔建升,段莉丽.浊度对荧光光谱法测定水体中叶绿素a的影响.理化检验-化学分册,2015,51(6):741-744.]
[22]Lv PY,Cui JS.Temperature effect on the determination of in situ fluorescence of chlorophyll-a in water.Environmental Monitoring in China,2015,31(2):130-134.[吕鹏翼,崔建升.温度对现场荧光法测定水体中叶绿素a的影响.中国环境监测,2015,31(2):130-134.]
