微弱荧光信号高灵敏大动态范围检测技术及应用

详细信息    查看全文 | 推荐本文 |

英文篇名：High sensitivity and large dynamic range detection technology of weak fluorescence signal and its application 作者：董鸣 ; 殷高方 ; 赵南京 ; 覃志松 ; 赵山 ; 马明俊 ; 肖雪 英文作者：DONG Ming;YIN Gaofang;ZHAO Nanjing;QIN Zhisong;ZHAO Shan;MA Mingjun;XIAO Xue;Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Sciences;University of Science and Technology of China;Key Laboratory of Optical Monitoring Technology for Environment,Anhui Province;Key Laboratory of Optical Monitoring Technology for Environment; 关键词：光谱学 ; 微弱荧光信号 ; 可变增益 ; 同步积分 ; 水体有机物 英文关键词：spectroscopy;;weak fluorescence signal;;variable gain;;synchronous integral;;organic matter in water 中文刊名：LDXU 英文刊名：Chinese Journal of Quantum Electronics 机构：中国科学院安徽光学精密机械研究所中国科学院环境光学与技术重点实验室;中国科学技术大学;安徽省环境光学检测技术重点实验室; 出版日期：2018-07-15 出版单位：量子电子学报 年：2018 期：v.35;No.183 基金：国家自然科学基金,61378041,31400317;; 国家重点研发计划项目,2016YFC1400600;; 安徽省科技重大专项,15CZZ04125;; 中科院STS计划项目,KFJ-SW-STS-170~~ 语种：中文; 页：LDXU201804002 页数：7 CN：04 ISSN：34-1163/TN 分类号：13-19

摘要

针对水体有机物离散三维荧光光谱测量,设计了基于可变增益与积分放大技术的微弱荧光信号高灵敏、大动态范围检测电路和控制软件,进行了实验验证并应用于水体有机物离散三维荧光在线测量系统。在电路设计中,自动程控CMOS多路模拟开关根据输入信号强度实现了前置增益放大器的调节,基于开关型电荷积分器实现了微弱脉冲荧光信号的同步积分放大。结果表明所设计电路最低检测电流为0.09μA,动态检测范围为0.09μA～0.21 mA,检测限提高了79.25倍。在水体有机物离散三维荧光测量系统的应用中,保证了系统的检测灵敏度,并提高了荧光信号的动态检测范围,实现了水体有机物的高精度测量。
        According to the discrete three-dimensional(3D) fluorescence spectrum detection of organic matter in water, the high sensitivity and large dynamic range detection circuit and control software of weak fluorescence signal based on the variable gain and integral amplification technology are designed. Experiment verification is carried out and it's applied to the on-line detection system for discrete 3D fluorescence of organic matter in water. In the designed circuit, according to the input signal intensity, automatic program control CMOS multi-channel analog switch is used to realize the adjustment of front gain amplifier. Based on the switching charge integrator, the synchronous integral amplification of weak pulse fluorescence signal is realized. Results show that the minimum detection current of the designed circuit is 0.09 μA, dynamic detection range is 0.09 μA～0.21 mA,the detection limit is increased by 79.25 times. In the application of discrete 3D fluorescence detection system of organic matter in water, the system detection sensitivity is guaranteed, and dynamic detection range of fluorescence signal is improved. The high precision measurement of organic matter in water is realized.

引文

[1]Du Zhiming,Pan Haiyan,Ding Yukui.Research on radiation characteristics of pulse Xe-lamp and saturated behaviors of photo-detector PbS[J].Transactions of Beijing Institute of Technology(北京理工大学学报),2005,25(8):722-725(in Chinese).
    [2]Zhou Weiye.Design of CMOS Variable Gain Amplifier with Wide Gain Dynamic Range(宽增益动态范围CMOS可变增益放大器设计)[D].Chengdu:Master Thesis of University of Electronic Science and Technology of China,2006(in Chinese).
    [3]Wang Ziqiang,Chi Baoyong,Wang Zhihua.An overview of CMOS variable gain amplifiers[J].Microelectronics(微电子学),2005,35(6):612-617(in Chinese).
    [4]Hu Tao,Si Hanying.Design and research of pre-amplifier circuit from a photoelectric detector[J].Electro-Optic Technology Application(光电技术应用),2010,25(1):52-55(in Chinese).
    [5]Zhou Shenghai,Wang Dongchen,Ma Jianzhong.Implementation of variable gain amplifier[J].Instrument Technique and Sensor(仪表技术与传感器),2001,7:32-34(in Chinese).
    [6]Gao Jinzhan.Detection of Weak Signals(微弱信号检测)[M].Beijing:Tsinghua University Press,2011:219-220(in Chinese).
    [7]Lu Weimin,Tian Shuxiang,Chen Zhencheng.Pulse data acquisition system based on integral amplifier IVC102[J].Chinese Medical Equipment Journal(医疗卫生装备),2015,36(2):17-20(in Chinese).