摘要
浮游植物有效光合反应中心浓度与其生长环境、生理状态密切相关,文中以生物膜能流理论为基础,基于初始荧光效率(F_0)和功能吸收截面(σPSII)的荧光动力学参数研究了浮游植物有效光合反应中心浓度分析方法。利用该方法对不同生长条件下的蛋白核小球藻进行了测试,结果表明:正常生理状态下,荧光动力学参数法与同化系数法分析结果具有良好的一致性,相关系数R~2达到0.999;非正常生理状态下,荧光动力学参数法较同化系数法更能准确反映浮游植物光合活性(F_v/F_m)和光合单元尺寸(n_(PSⅡ))引起的有效光合反应中心浓度的变化;在短期胁迫条件下,荧光动力学参数法分析结果与F_v/F_m相关系数R~2可达0.920;在长期光照胁迫条件下的分析结果也能反映光照变化引起的浮游植物n_(PSⅡ)变化信息,且与已有研究成果相符。研究结果为浮游植物有效光合反应中心浓度的准确测量提供了一种新方法。
Phytoplankton concentration of functional reaction center is closely related to its growth environment and physiological state.A new method is used to analyze the phytoplankton concentration of functional reaction center based on the biological energy flow theory,in other words,based on the fluorescence kinetic parameters such as initial fluorescence efficiency(F_0)and function absorption cross section(σPSII)is presented in this paper.The fluorescence parameters of Cholorella pyrenoidosa are measured under different conditions.The results show that there is a good agreement between the result of fluorescence kinetic parameter method and that of assimilation coefficient method under normal physiological conditions,and the correlation coefficient is 0.999.Compared with the assimilation coefficient method,the fluorescence kinetic parameter method is more accurate to analyze the changes in phytoplankton concentration of functional reaction center induced by photosynthetic activity(F_v/F_m)and photosynthetic unit sizes(n_(PSⅡ))under abnormal physiological conditions.The phytoplankton concentration of functional reaction center obtained by the fluorescence kinetic parameter method is correlated with F_v/F_m,and the correlation coefficient is 0.920 under the short-term stress condition.The changes of nPSIIcaused by light can be measured by the fluorescence kinetic parameter method under the long-term light stress condition,which are consistent with the existing research results.This new method can accurately measure phytoplankton concentration of functional reaction center.
引文
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