摘要
[目的]建立一种能同时测定7%炔草酯·氟唑磺隆·甲基二磺隆可分散油悬浮剂中3种有效成分以及解毒喹、吡唑解草酯2种解毒剂的高效液相色谱检测方法。[方法]采用反相高效液相色谱法,使用C_(18)色谱柱和UV检测器,以乙腈和0.5%磷酸水溶液(体积比55∶45)为流动相,流速为1.0 mL/min,在225 nm检测波长下,对7%炔草酯·氟唑磺隆·甲基二磺隆可分散油悬浮剂中炔草酯、氟唑磺隆和甲基二磺隆及解毒剂解毒喹和吡唑解草酯5种活性成分同时进行含量测定。[结果]在上述分析条件下,炔草酯、氟唑磺隆和甲基二磺隆及解毒剂解毒喹和吡唑解草酯5种活性成分的标准偏差小,变异系数小,平均回收率接近100%,同时具有良好的线性关系。[结论]该分析方法能一次分离7%炔草酯·氟唑磺隆·甲基二磺隆可分散油悬浮剂中5种活性成分,简便快捷,分离效果好,精密度和准确度高,线性关系好,是理想的分析方法。
[Aims] A method for simultaneous analysis of three active ingredients include clodinafop,flucarbazone-sodium and mesosulfuron-methyl, two antidotes include cloquintocet-mexyl and mefenpyr-diethyl in clodinafop·flucarbazone-sodium·mesosulfuron-methyl 7% OD by HPLC was established. [Methods] The separation and quantitative analysis of clodinafop, flucarbazone-sodium, mesosulfuron-methyl, cloquintocet-mexyl and mefenpyr-diethyl in clodinafop·flucarbazone-sodium·mesosulfuron-methyl 7% OD were conducted by HPLC and UV detector with the mixture of acetonitrile and 0.5% phosphoric acid solution(55∶45, by vol at 1.0 mL/min) as the mobile phase, the ODS C18 column as fix phase at 225 nm. [Results] The linear correlation coefficients for clodinafop,flucarbazone-sodium, mesosulfuron-methyl, cloquintocet-mexyl and mefenpyr-diethyl were good, the standard deviations and the variation coefficients were small, the average recoveries were close to 100%. [Conclusions] This analytical method can separate the five active ingredients in clodinafop·flucarbazone-sodium·mesosulfuron-methyl 7%OD at one time. It is simple, quick, efficient, accurate, and has a good linearity, which is an ideal analytical method for clodinafop·flucarbazone-sodium·mesosulfuron-methyl 7% OD.
引文
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