摘要
以霉变鲜剥大蒜中分离纯化的致腐霉菌(桔青霉、匍枝根霉、赤霉和赭曲霉)为实验菌种,探究不同臭氧处理对其抑制作用。采用不同浓度、不同时间和不同温度臭氧处理4种霉菌,通过测定致死率、菌丝生长抑制率、电解质溶出率、蛋白质溶出率和菌体微观形态来评价臭氧的抑菌效果。结果表明,臭氧对桔青霉、匍枝根霉、赤霉和赭曲霉4种霉菌的最佳处理条件分别为20℃下6μL/L的臭氧处理20 min、25℃下8μL/L的臭氧处理20 min、25℃下6μL/L的臭氧处理20 min、25℃下8μL/L的臭氧处理15min。
This paper studied the inhibitory effects of different ozone treatments on spoilage molds(Penicillium citrinum, Rhizopus stolonifer, Gibberella intermedia, Aspergillus ochraceus) which were separated and purified from mildew garlic. After treating four kinds of spoilage molds with different ozone concentration, different time and different temperature, the death rate, mycelial growth inhibition rate, electrolyte dissolution rate, protein dissolution rate and micromorphology of the molds were analyzed to study the inhibition effect of ozone. The results indicated that the optimum treatment conditions of ozone on Penicillium citrinum, Rhizopus stolonifer, Gibberella intermedia and Aspergillus ochraceus were 6μL/L ozone treatment for 20min at 20℃, 8μL/L ozone treatment for 20min at 25℃, 6μL/L ozone treatment for 20min at 25℃ and 8μL/L ozone treatment for 15min at 25℃, respectively.
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